Multiple Amidated Neuropeptides Are Required for Normal Circadian Locomotor Rhythms in Drosophila

In Drosophila, the amidated neuropeptide pigment dispersing factor (PDF) is expressed by the ventral subset of lateral pacemaker neurons and is required for circadian locomotor rhythms. Residual rhythmicity in pdf mutants likely reflects the activity of other neurotransmitters. We asked whether othe...

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Bibliographic Details
Published in:	The Journal of neuroscience Vol. 21; no. 17; pp. 6673 - 6686
Main Authors:	Taghert, Paul H, Hewes, Randall S, Park, Jae H, O'Brien, Martha A, Han, Mei, Peck, Molly E
Format:	Journal Article
Language:	English
Published:	United States Soc Neuroscience 01-09-2001 Society for Neuroscience
Subjects:	Amides - metabolism Animals Behavior, Animal - physiology Central Nervous System - cytology Central Nervous System - physiology Circadian Rhythm - physiology DNA-Binding Proteins Drosophila Drosophila Proteins Enhancer Elements, Genetic - physiology Fungal Proteins - genetics Genes, Lethal Genes, Reporter Locomotion - physiology Male Mixed Function Oxygenases - biosynthesis Mixed Function Oxygenases - genetics Mosaicism - genetics Multienzyme Complexes - biosynthesis Multienzyme Complexes - genetics Neurons - metabolism Neuropeptides - biosynthesis Neuropeptides - genetics Neuropeptides - metabolism Organ Specificity - genetics Promoter Regions, Genetic - genetics Saccharomyces cerevisiae Proteins Transcription Factors - genetics
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Summary:	In Drosophila, the amidated neuropeptide pigment dispersing factor (PDF) is expressed by the ventral subset of lateral pacemaker neurons and is required for circadian locomotor rhythms. Residual rhythmicity in pdf mutants likely reflects the activity of other neurotransmitters. We asked whether other neuropeptides contribute to such auxiliary mechanisms. We used the gal4/UAS system to create mosaics for the neuropeptide amidating enzyme PHM; amidation is a highly specific and widespread modification of secretory peptides in Drosophila. Three different gal4 drivers restricted PHM expression to different numbers of peptidergic neurons. These mosaics displayed aberrant locomotor rhythms to degrees that paralleled the apparent complexity of the spatial patterns. Certain PHM mosaics were less rhythmic than pdf mutants and as severe as per mutants. Additional gal4 elements were added to the weakly rhythmic PHM mosaics. Although adding pdf-gal4 provided only partial improvement, adding the widely expressed tim-gal4 largely restored rhythmicity. These results indicate that, in Drosophila, peptide amidation is required for neuropeptide regulation of behavior. They also support the hypothesis that multiple amidated neuropeptides, acting upstream, downstream, or in parallel to PDF, help organize daily locomotor rhythms.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0270-6474 1529-2401
DOI:	10.1523/JNEUROSCI.21-17-06673.2001