ATP-dependent nucleosome reconfiguration and transcriptional activation from preassembled chromatin templates

GAL4-VP16-mediated nucleosome reconfiguration and transcriptional activation were observed with preassembled chromatin templates that contained regular and physiological nucleosome spacing. Both processes were dependent on adenosine triphosphate (ATP), although binding of GAL4-VP16 to the chromatin...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) Vol. 266; no. 5193; pp. 2007 - 2011
Main Authors:	Pazin, M.J, Kamakaka, R.T, Kadonaga, J.T
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for the Advancement of Science 23-12-1994 American Association for the Advancement of Science The American Association for the Advancement of Science
Subjects:	adenosine triphosphate Adenosine Triphosphate - metabolism Animals Binding sites Biochemical genetics Biological and medical sciences Chromatin Chromatin - chemistry Chromatin - metabolism DNA DNA - metabolism DNA conformation DNA-Binding Proteins Drosophila Drosophila melanogaster Fundamental and applied biological sciences. Psychology Fungal Proteins - metabolism gal4 protein Genes Genetic regulation Genetic transcription Histones Inhibition Models, Genetic Molecular and cellular biology Molecular genetics Nucleosomes Nucleosomes - chemistry Nucleosomes - metabolism Plasmids Regulation Repression Saccharomyces cerevisiae Proteins Templates, Genetic Tin Topology Trans-Activators - metabolism transcription (genetics) Transcription factors Transcription Factors - metabolism Transcription. Transcription factor. Splicing. Rna processing Transcriptional Activation transcriptional activators Yeast Molecular arrangement Chromatin Regulation(control) Transcription Nucleosome Transcription factor ATP In vitro
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Summary:	GAL4-VP16-mediated nucleosome reconfiguration and transcriptional activation were observed with preassembled chromatin templates that contained regular and physiological nucleosome spacing. Both processes were dependent on adenosine triphosphate (ATP), although binding of GAL4-VP16 to the chromatin was ATP-independent. Factor-mediated nucleosome reconfiguration was not, however, sufficient for transcriptional activation. These experiments recreate in vitro the active participation of nucleosomal cores in the regulation of transcription that occurs in vivo, and they suggest a multistep pathway for transcriptional activation in which factor- and ATP-dependent nucleosome reconfiguration is followed by facilitation by the DNA-bound activator of transcription from the repressed chromatin template.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.7801129