Comparative HMG-box sequences of the SRY gene between sheep, cattle and goats

In mammals, sexual differentiation of the gonads during development depends on the presence of the Y chromosome. The testis-determining gene lies on the Y chromosome, and has been delimited in human to a 35-kilobase region near the pseudoautosomal boundary. SRY is a gene located in this region and b...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 21; no. 11; p. 2772
Main Authors:	Payen, Emmanuel J., Cotinot, Corinne Y.
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 11-06-1993
Subjects:	Amino Acid Sequence Animals Base Sequence Biological and medical sciences Bovidae Cattle - genetics Conserved Sequence DNA-Binding Proteins - metabolism Female Fundamental and applied biological sciences. Psychology Genes. Genome Goats - genetics High Mobility Group Proteins - genetics High Mobility Group Proteins - metabolism Humans Life Sciences Male Molecular and cellular biology Molecular genetics Molecular Sequence Data Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Sex Differentiation Sheep - genetics Y Chromosome Sequence specificity Bovine Nucleotide sequence Sequence alignment Caprine Gene organization Y-Chromosome Vertebrata Mammalia Sex determination Sheep Artiodactyla Ungulata
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Summary:	In mammals, sexual differentiation of the gonads during development depends on the presence of the Y chromosome. The testis-determining gene lies on the Y chromosome, and has been delimited in human to a 35-kilobase region near the pseudoautosomal boundary. SRY is a gene located in this region and both conserved during evolution and Y chromosome specific in a number of mammals. The predicted amino acid sequence of SRY contains a domain characterized by a homology with hUBF, the Mc protein of the fission yeast Schizosaccharomyces pombe , the high-mobility-group protein HMG sub(1) and the lymphoid-specific enhancer binding factor LEF-1. This domain of 70-residues termed HMG box is characteristic of DNA-binding proteins. This conserved motif represents a functional protein domain necessary for DNA-binding activity of SRY. Until now, all mutations in the SRY gene associated with sex inversion have been located within the HMG box. Using oligonucleotide primers within the HMG box in the human SRY sequence, genomic DNA from males and females of the Bovidae family was amplified by the polymerase chain reaction (PCR). The resulting male specific amplified fragments were sequenced after assymetric PCR. The 200 bases pairs of the sequence of each PCR product have been determined. The nucleic acid comparison reveal a very high degree of conservation of the SRY - HMG box within the Bovidae family.
Bibliography:	ark:/67375/HXZ-WVR2N9L5-J istex:CF97BE6D1D895A294FEF8CF99D54B07F64D1E1DD ArticleID:21.11.2772 To whom correspondence should be addressed ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/21.11.2772