Search Results - "Pawate, S"

Presentations and outcomes of neurosarcoidosis: a study of 54 cases by Pawate, S., Moses, H., Sriram, S.

“…Objective: To report on the clinical presentations, laboratory abnormalities, treatment and outcomes in 54 patients with neurosarcoidosis (NS). Background:…”
Get full text

Extended interval dosing of natalizumab in multiple sclerosis by Zhovtis Ryerson, L, Frohman, T C, Foley, J, Kister, I, Weinstock-Guttman, B, Tornatore, C, Pandey, K, Donnelly, S, Pawate, S, Bomprezzi, R, Smith, D, Kolb, C, Qureshi, S, Okuda, D, Kalina, J, Rimler, Z, Green, R, Monson, N, Hoyt, T, Bradshaw, M, Fallon, J, Chamot, E, Bucello, M, Beh, S, Cutter, G, Major, E, Herbert, J, Frohman, E M

“…BackgroundNatalizumab (NTZ), a monoclonal antibody to human α4β1/β7 integrin, is an effective therapy for multiple sclerosis (MS), albeit associated with…”
Get full text

Fabrication of X-ray compatible microfluidic platforms for protein crystallization by Guha, Sudipto, Perry, Sarah L., Pawate, Ashtamurthy S., Kenis, Paul J.A.

“…This paper reports a method for fabricating multilayer microfluidic protein crystallization platforms using different materials to achieve X-ray transparency…”
Get full text

A Method of Cryoprotection for Protein Crystallography by Using a Microfluidic Chip and Its Application for in Situ X‑ray Diffraction Measurements by Maeki, Masatoshi, Pawate, Ashtamurthy S, Yamashita, Kenichi, Kawamoto, Masahide, Tokeshi, Manabu, Kenis, Paul J. A, Miyazaki, Masaya

“…We demonstrate a seamless and contactless method from protein crystallization to X-ray analysis using a microfluidic chip with the aim of obtaining a complete…”
Get full text

X‑ray Transparent Microfluidic Chip for Mesophase-Based Crystallization of Membrane Proteins and On-Chip Structure Determination by Khvostichenko, Daria S, Schieferstein, Jeremy M, Pawate, Ashtamurthy S, Laible, Philip D, Kenis, Paul J. A

“…Crystallization from lipidic mesophase matrices is a promising route to diffraction-quality crystals and structures of membrane proteins. The microfluidic…”
Get full text

A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction by Perry, Sarah L, Guha, Sudipto, Pawate, Ashtamurthy S, Bhaskarla, Amrit, Agarwal, Vinayak, Nair, Satish K, Kenis, Paul J A

“…We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray…”
Get more information

An X-ray transparent microfluidic platform for screening of the phase behavior of lipidic mesophases by Khvostichenko, Daria S, Kondrashkina, Elena, Perry, Sarah L, Pawate, Ashtamurthy S, Brister, Keith, Kenis, Paul J A

“…Lipidic mesophases are a class of highly ordered soft materials that form when certain lipids are mixed with water. Understanding the relationship between the…”
Get more information

A Mutation in Subunit I of Cytochrome Oxidase from Rhodobacter sphaeroides Results in an Increase in Steady-State Activity but Completely Eliminates Proton Pumping by Pawate, Ashtamurthy S, Morgan, Joel, Namslauer, Andreas, Mills, Denise, Brzezinski, Peter, Ferguson-Miller, Shelagh, Gennis, Robert B

“…The heme−copper oxidases convert the free energy liberated in the reduction of O2 to water into a transmembrane proton electrochemical potential (protonmotive…”
Get full text

Towards time-resolved serial crystallography in a microfluidic device by Pawate, Ashtamurthy S., Šrajer, Vukica, Schieferstein, Jeremy, Guha, Sudipto, Henning, Robert, Kosheleva, Irina, Schmidt, Marius, Ren, Zhong, Kenis, Paul J. A., Perry, Sarah L.

“…Serial methods for crystallography have the potential to enable dynamic structural studies of protein targets that have been resistant to single‐crystal…”
Get full text

Redox-Coupled Proton Translocation in Biological Systems: Proton Shuttling in Cytochrome c Oxidase by Namslauer, Andreas, Pawate, Ashtamurthy S., Gennis, Robert B., Brzezinski, Peter

“…In the respiratory chain free energy is conserved by linking the chemical reduction of dioxygen to the electrogenic translocation of protons across a membrane…”
Get full text

Transmembrane Charge Separation during the Ferryl-oxo → Oxidized Transition in a Nonpumping Mutant of Cytochrome c Oxidase by Siletsky, Sergey A, Pawate, Ashtamurthy S, Weiss, Kara, Gennis, Robert B, Konstantinov, Alexander A

“…The N139D mutant of cytochrome c oxidase from Rhodobacter sphaeroides retains full steady state oxidase activity but completely lacks proton translocation…”
Get full text

Controlled Uncoupling and Recoupling of Proton Pumping in Cytochrome c Oxidase by Brändén, Gisela, Pawate, Ashtamurthy S., Gennis, Robert B., Brzezinski, Peter

“…Cytochrome c oxidase (CcO) is the terminal enzyme of the respiratory chain and couples energetically the reduction of oxygen to water to proton pumping across…”
Get full text

Isolated longitudinal myelitis: a report of six cases by Pawate, S, Sriram, S

“…Study design: Retrospective chart review. Objective To report six patients with isolated longitudinal myelitis (LM). Setting: Outpatients at Multiple Sclerosis…”
Get full text

X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization by Schieferstein, Jeremy M., Pawate, Ashtamurthy S., Sun, Chang, Wan, Frank, Sheraden, Paige N., Broecker, Jana, Ernst, Oliver P., Gennis, Robert B., Kenis, Paul J. A.

“…Elucidating and clarifying the function of membrane proteins ultimately requires atomic resolution structures as determined most commonly by X-ray…”
Get full text

Polymeric microfluidic continuous flow mixer combined with hyperspectral FT-IR imaging for studying rapid biomolecular events by Jang, Hyukjin, Pawate, Ashtamurthy S, Bhargava, Rohit, Kenis, Paul J A

“…Early reaction intermediates in protein folding, such as those resulting in β-amyloid formation due to transient misfolding, emerge within a few hundred…”
Get more information

X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds by Schieferstein, Jeremy M, Pawate, Ashtamurthy S, Varel, Michael J, Guha, Sudipto, Astrauskaite, Ieva, Gennis, Robert B, Kenis, Paul J A

“…Crystallization of membrane proteins is a critical step for uncovering atomic resolution 3-D structures and elucidating structure-function relationships…”
Get more information

Development of microfluidic platform that enables ‘on-chip’ imaging of cells exposed to shear stress and cyclic stretch by Sinclair, Whitney E., Pawate, Ashtamurthy S., Larry, Ty’Nya A., Schieferstein, Jeremy M., Whittenberg, Joseph J., Leckband, Deborah E., Kenis, Paul J. A.

“…Here, we describe the development of an organ-on-a-chip platform that enables ex vivo studies under active physio-mechanical stress by applying hydrodynamic…”
Get full text

In situ serial Laue diffraction on a microfluidic crystallization device by Perry, Sarah L., Guha, Sudipto, Pawate, Ashtamurthy S., Henning, Robert, Kosheleva, Irina, Srajer, Vukica, Kenis, Paul J. A., Ren, Zhong

“…Renewed interest in room‐temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial…”
Get full text

Chemical Analysis of Drug Biocrystals: A Role for Counterion Transport Pathways in Intracellular Drug Disposition by Keswani, Rahul K, Baik, Jason, Yeomans, Larisa, Hitzman, Chuck, Johnson, Allison M, Pawate, Ashtamurthy S, Kenis, Paul J. A, Rodriguez-Hornedo, Nair, Stringer, Kathleen A, Rosania, Gus R

“…In mammals, highly lipophilic small molecule chemical agents can accumulate as inclusions within resident tissue macrophages. In this context, we characterized…”
Get full text

Non‐Aqueous Primary Li–Air Flow Battery and Optimization of its Cathode through Experiment and Modeling by Kim, Byoungsu, Takechi, Kensuke, Ma, Sichao, Verma, Sumit, Fu, Shiqi, Desai, Amit, Pawate, Ashtamurthy S., Mizuno, Fuminori, Kenis, Paul J. A.

“…A primary Li–air battery has been developed with a flowing Li‐ion free ionic liquid as the recyclable electrolyte, boosting power capability by promoting…”
Get full text