Search Results - "Pavlov, M Y"

Novel Roles for Classical Factors at the Interface between Translation Termination and Initiation by Karimi, Reza, Pavlov, Michael Y, Buckingham, Richard H, Ehrenberg, Måns

“…The pathway of bacterial ribosome recycling following translation termination has remained obscure. Here, we elucidate two essential steps and describe the…”
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Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs by Pavlov, M.Y., Freistroffer, D.V., Ehrenberg, M.

“…A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro translation of matrix-coupled…”
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Purification of active Escherichia coli ribosome recycling factor (RRF) from an osmo-regulated expression system by MacDougall, J., Holst-Hansen, P., Mortensen, K.K., Freistroffer, D.V., Pavlov, M.Y., Ehrenberg, M., Buckingham, R.H.

“…Ribosome release factor (RRF) from Escherichia coli was overproduced from an osmo-expression vector. More than 40% of cell protein was RRF after 6 h of…”
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Rate of translation of natural mRNAs in an optimized in vitro system by Pavlov, M Y, Ehrenberg, M

“…We report results on in vitro translation of an mRNA coding for elongation factor TuB which was in vitro transcribed from the tufB gene from Escherichia coli…”
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Inhibition of cholesterol synthesis and esterification regulates high density lipoprotein interaction with isolated epithelial cells of human small intestine by Sviridov, D D, Pavlov, M Y, Safonova, I G, Repin, V S, Smirnov, V N

“…The effect of two inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, lovastatin and monacolin L, and an inhibitor of acyl coenzyme…”
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Fast recycling of Escherichia coli ribosomes requires both ribosome recycling factor (RRF) and release factor RF3 by Pavlov, Michael Yu, Freistroffer, David V., MacDougall, Jane, Buckingham, Richard H., Ehrenberg, Måns

“…A complete translation system has been assembled from pure initiation, elongation and termination factors as well as pure aminoacyl‐tRNA synthetases. In this…”
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Initiation factors IF1 and IF2 synergistically remove peptidyl-tRNAs with short polypeptides from the P-site of translating Escherichia coli ribosomes by Karimi, Reza, Pavlov, Michael Yu, Heurgué-Hamard, Valérie, Buckingham, Richard H., Ehrenberg, Måns

“…A novel function of initiation factors IF1 and IF2 in Escherichia coli translation has been identified. It is shown that these factors efficiently catalyse…”
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Inhibition of cholesterol synthesis by lovastatin tested on six human cell types in vitro by SVIRIDOV, D. D, SAFONOVA, I. G, PAVLOV, M. Y, KOSYKH, V. A, PODREZ, E. A, ANTONOV, A. S, FUKI, I. V, REPIN, V. S

“…Lovastatin (mevinolin) caused a strong and dose-dependent inhibition of cholesterol synthesis in six types of cultured human cells. Fifty percent inhibition of…”
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New model to study cholesterol uptake in the human intestine in vitro by Sviridov, D.D., Safonova, I.G., Nano, J.L., Pavlov, M.Y., Rampal, P, Repin, V.S., Smirnov, V.N.

“…A new model to study cholesterol uptake in the human intestine in vitro is described. Human small intestine organ cultures were incubated with mixed micelles…”
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A direct estimation of the context effect on the efficiency of termination by Pavlov, Michael Yu, Freistroffer, David V., Dincbas, Vildan, MacDougall, Jane, Buckingham, Richard H., Ehrenberg, Måns

“…An in vitro assay in which terminating Escherichia coli ribosomes with different stop signals in the A-site compete for a limited amount of a release factor…”
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Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site by Pavlov, Michael Yu, Freistroffer, David V, Heurgué-Hamard, Valérie, Buckingham, Richard H, Ehrenberg, Måns

“…The dependence of the rate of ribosomal recycling (from initiation via protein elongation and termination, and then back to initiation) on the concentrations…”
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The Role of "Russian Question" in The Resolution of Modern Global Conflicts by Pavlov Y. M., Pavlov, Y. M.

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Binding of SecB to Ribosome-Bound Polypeptides has the Same Characteristics as Binding to Full-Length, Denatured Proteins by Randall, Linda L., Topping, Traci B., Simon J. S. Hardy, Pavlov, Michael Y., Freistroffer, David V., Ehrenberg, Mans

“…The interaction of the chaperone SecB with ribosome-bound polypeptides that are in the process of elongation has been studied using an in vitro protein…”
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Mutations in conserved regions of ribosomal RNAs decrease the productive association of peptide-chain release factors with the ribosome during translation termination by Arkov, Alexey L., Freistroffer, David V., Pavlov, Michael Yu, Ehrenberg, Måns, Murgola, Emanuel J.

“…Early studies provided evidence that peptide-chain release factors (RFs) bind to both ribosomal subunits and trigger translation termination. Although many…”
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Initiation of Escherichia coli ribosomes on matrix coupled mRNAs studied by optical biosensor technique by Karlsson, Maria, Pavlov, Michael Yu, Malmqvist, Magnus, Persson, Björn, Ehrenberg, Måns

“…The optical biosensor technique, based on the surface plasmon resonance (SPR) phenomenon, has been used to study the initiation of protein synthesis by E. coli…”
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Inhibition of cholesterol synthesis by lovastatin tested on six human cell typesin vitro by Sviridov, D. D., Safonova, I. G., Pavlov, M. Y., Kosykh, V. A., Podrez, E. A., Antonov, A. S., Fuki, I. V., Repin, V. S.

“…Lovastatin (mevinolin) caused a strong and dose‐dependent inhibition of cholesterol synthesis in six types of cultured human cells. Fifty percent inhibition of…”
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Ribosome Rescue by tmRNA Requires Truncated mRNAs by Ivanova, Natalia, Pavlov, Michael Y., Felden, Brice, Ehrenberg, Måns

“…tmRNA targets ribosomes, stalled either on truncated mRNAs or on mRNAs with slowly read sense or stop codons, tags the newly synthesized peptide chains for…”
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Studies on the proteins involved in the interaction of high-density lipoprotein with isolated human small intestine epithelial cells by Sviridov, D.D., Ehnholm, C., Tenkanen, H., Pavlov, M.Yu, Safonova, I.G., Repin, V.S.

“…Treatment of 125I-labelled high-density lipoprotein ([ 125I]HDL 3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a…”
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The roles of initiation factor 2 and guanosine triphosphate in initiation of protein synthesis by Ehrenberg, Måns, Antoun, Ayman, Pavlov, Michael Y, Andersson, Kerstin, Tenson, Tanel

“…The role of IF2 from Escherichia coli was studied in vitro using a system for protein synthesis with purified components. Stopped flow experiments with light…”
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Release factor RF3 in E-coli accelerates the dissociation of release factors RF1 and RF2 from the ribosome in a GTP-dependent manner by Freistroffer, DV, Pavlov, MY, MacDougall, J, Buckingham, RH, Ehrenberg, M

“…Ribosomes complexed with synthetic mRNA and peptidyl-tRNA, ready for peptide release, were purified by gel filtration and used to study the function of release…”
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