Search Results - "Pavalko, Fredrick M"

Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells by Day, Richard N, Day, Kathleen H, Pavalko, Fredrick M

“…Earlier, we proposed the "mechanosome" concept as a testable model for understanding how mechanical stimuli detected by cell surface adhesion molecules are…”
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Focal Adhesion Kinase Is Important for Fluid Shear Stress‐Induced Mechanotransduction in Osteoblasts by Young, Suzanne RL, Gerard‐O'Riley, Rita, Kim, Jae‐Beom, Pavalko, Fredrick M

“…Mechanical loading of bone is important for maintenance of bone mass and structural stability of the skeleton. When bone is mechanically loaded, movement of…”
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Mechanical loading in osteocytes induces formation of a Src/Pyk2/MBD2 complex that suppresses anabolic gene expression by Hum, Julia M, Day, Richard N, Bidwell, Joseph P, Wang, Yingxiao, Pavalko, Fredrick M

“…Mechanical stimulation of the skeleton promotes bone gain and suppresses bone loss, ultimately resulting in improved bone strength and fracture resistance. The…”
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Lrp4 Mediates Bone Homeostasis and Mechanotransduction through Interaction with Sclerostin In Vivo by Bullock, Whitney A., Hoggatt, April M., Horan, Daniel J., Elmendorf, Andrew J., Sato, Amy Y., Bellido, Teresita, Loots, Gabriela G., Pavalko, Fredrick M., Robling, Alexander G.

“…Wnt signaling plays a key role in regulating bone remodeling. In vitro studies suggest that sclerostin's inhibitory action on Lrp5 is facilitated by the…”
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Non-overlapping functions for Pyk2 and FAK in osteoblasts during fluid shear stress-induced mechanotransduction by Young, Suzanne R L, Hum, Julia M, Rodenberg, Eric, Turner, Charles H, Pavalko, Fredrick M

“…Mechanotransduction, the process by which cells convert external mechanical stimuli such as fluid shear stress (FSS) into biochemical changes, plays a critical…”
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Monitoring biosensor activity in living cells with fluorescence lifetime imaging microscopy by Hum, Julia M, Siegel, Amanda P, Pavalko, Fredrick M, Day, Richard N

“…Live-cell microscopy is now routinely used to monitor the activities of the genetically encoded biosensor proteins that are designed to directly measure…”
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Mechanotransduction and functional response of the skeleton to physical stress: the mechanisms and mechanics of bone adaptation by Turner, C H, Pavalko, F M

“…The skeleton's primary mechanical function is to provide rigid levers for muscles to act against as they hold the body upright in defiance of gravity. Many…”
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Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions by Pavalko, F. M., Chen, N. X., Turner, C. H., Burr, D. B., Atkinson, S., Hsieh, Y. F., Qiu, J., Duncan, R. L.

“…Mechanical stimulation of bone induces new bone formation in vivo and increases the metabolic activity and gene expression of osteoblasts in culture. We…”
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HMGB1 expression and release by bone cells by Charoonpatrapong, Kanokwan, Shah, Rita, Robling, Alexander G., Alvarez, Marta, Clapp, D. Wade, Chen, Shi, Kopp, Ryan P., Pavalko, Fredrick M., Yu, Jun, Bidwell, Joseph P.

“…Immune and bone cells are functionally coupled by pro‐inflammatory cytokine intercellular signaling networks common to both tissues and their crosstalk may…”
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Nmp4/CIZ contributes to fluid shear stress induced MMP-13 gene induction in osteoblasts by Charoonpatrapong-Panyayong, Kanokwan, Shah, Rita, Yang, Jieping, Alvarez, Marta, Pavalko, Fredrick M., Gerard-O'Riley, Rita, Robling, Alexander G., Templeton, Evan, Bidwell, Joseph P.

“…The expression of matrix metalloproteinase‐13 (MMP‐13), involved in bone turnover, is elevated in stretched MC3T3‐E1 osteoblast‐like cells. Strain‐mediated…”
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PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells by Reissaus, Christopher A., Day, Kathleen H., Mirmira, Raghavendra G., Dunn, Kenneth W., Pavalko, Fredrick M., Day, Richard N.

“…We report the use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measure the activities of two different biosensor…”
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Osteoblasts and osteocytes respond differently to oscillatory and unidirectional fluid flow profiles by Ponik, Suzanne M., Triplett, Jason W., Pavalko, Fredrick M.

“…Bone cells subjected to mechanical loading by fluid shear stress undergo significant architectural and biochemical changes. The models of shear stress used to…”
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Expression of a Degradation‐Resistant β‐Catenin Mutant in Osteocytes Protects the Skeleton From Mechanodeprivation‐Induced Bone Wasting by Bullock, Whitney A, Hoggatt, April M, Horan, Daniel J, Lewis, Karl J, Yokota, Hiroki, Hann, Steven, Warman, Matthew L, Sebastian, Aimy, Loots, Gabriela G, Pavalko, Fredrick M, Robling, Alexander G

“…ABSTRACT Mechanical stimulation is a key regulator of bone mass, maintenance, and turnover. Wnt signaling is a key regulator of mechanotransduction in bone,…”
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Estrogen receptor-β regulates mechanical signaling in primary osteoblasts by Castillo, Alesha B, Triplett, Jason W, Pavalko, Fredrick M, Turner, Charles H

“…Mechanical loading is an important regulator in skeletal growth, maintenance, and aging. Estrogen receptors have a regulatory role in mechanically induced bone…”
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Hydroxy-Safflower Yellow A inhibits the TNFR1-Mediated Classical NF-κB Pathway by Inducing Shedding of TNFR1 by Wang, Haifang, Liu, Jinlian, Yang, Yuejin, Cao, Qingwen, Huo, Xueping, Ma, Shuhui, Hu, Jun, Pavalko, Fredrick M., Liu, Qinshe

“…Hydroxy‐safflower yellow A (HSYA) is the major active component of safflower, a traditional Asia herbal medicine well known for its cardiovascular protective…”
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Hydroxy-Safflower Yellow A inhibits the TNFR1-Mediated Classical NF-[kappa]B Pathway by Inducing Shedding of TNFR1 by Wang, Haifang, Liu, Jinlian, Yang, Yuejin, Cao, Qingwen, Huo, Xueping, Ma, Shuhui, Hu, Jun, Pavalko, Fredrick M, Liu, Qinshe

“…Hydroxy-safflower yellow A (HSYA) is the major active component of safflower, a traditional Asia herbal medicine well known for its cardiovascular protective…”
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Formation of focal adhesions on fibronectin promotes fluid shear stress induction of COX-2 and PGE2 release in MC3T3-E1 osteoblasts by Ponik, Suzanne M, Pavalko, Fredrick M

“…Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana 46202 Submitted 24 November 2003 ; accepted in…”
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Activation of NF-κB by fluid shear stress, but not TNF-α, requires focal adhesion kinase in osteoblasts by Young, Suzanne R.L, Gerard-O'Riley, Rita, Harrington, Maureen, Pavalko, Fredrick M

“…Abstract When bone is mechanically loaded fluid shear stress (FSS) is generated as a result of the movement of interstitial fluid across the membranes of…”
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Mechanosomes carry a loaded message by Bidwell, Joseph P, Pavalko, Fredrick M

“…Understanding the molecular mechanisms that mediate the response of cells to mechanical stimuli, the process known as mechanotransduction, has emerged as a…”
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Disruption of alpha-actinin-integrin interactions at focal adhesions renders osteoblasts susceptible to apoptosis by Triplett, Jason W, Pavalko, Fredrick M

“…Maintenance of bone structural integrity depends in part on the rate of apoptosis of bone-forming osteoblasts. Because substrate adhesion is an important…”
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