Effects of Aloe vera extract on growth, viability, ultrastructure and expression of mRNA for antioxidant enzymes in bovine secondary follicles cultured in vitro

Effects of Aloe vera extract on growth, viability, ultrastructure and expression of mRNA for antioxidant enzymes in bovine secondary follicles cultured in vitro

This study aimed to investigate the effects of Aloe vera extract on follicular growth, viability, ultrastructure, and mRNA levels for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase 1 (GPX1) and peroxiredoxin 6 (PRDX6) in bovine secondary follicles cultured in vitro. To this end,...

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Published in:Animal reproduction science Vol. 247; p. 107078
Main Authors: Azevedo, Venância A.N., Barroso, Pedro A.A., Vasconcelos, Erlândia M., Costa, Francisco C., Assis, Ernando I.T., Silva, Bianca R., Paulino, Laís R.M., Silva, Anderson W.B., Donato, Mariana M.A., Peixoto, Cristina A., Silva, José R.V., Souza, Ana L.P.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-12-2022
Subjects:
Aloe - metabolism
Aloe vera
Animals
Antioxidant
Antioxidants - pharmacology
Cattle
Cow
In vitro culture
Plant Extracts - pharmacology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Secondary follicle
Superoxide Dismutase
Cow
Aloe vera
In vitro culture
Antioxidant
Secondary follicle
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Summary:This study aimed to investigate the effects of Aloe vera extract on follicular growth, viability, ultrastructure, and mRNA levels for superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase 1 (GPX1) and peroxiredoxin 6 (PRDX6) in bovine secondary follicles cultured in vitro. To this end, secondary follicles were mechanically isolated from the ovarian cortex and cultured at 38.5 °C, with 5% CO2 in air, for 18 days in TCM-199+ alone or supplemented with 2.5%, 5.0%, 10.0% and 20.0% Aloe vera extract. Follicular growth, morphology and antrum formation were evaluated every 6 days, while ultrastructure was evaluated at the end of culture. Analysis of viability was performed by calcein-AM and ethidium homodimer-1, while mRNA levels for SOD, CAT, GPX1 and PRDX6 were evaluated by real-time PCR at the end of culture. The results show that follicles cultured with 2.5% Aloe vera had increased the rate of antrum formation, while 2.5% and 5.0% Aloe vera improved follicular viability rate. Follicles cultured with 2.5% and 10.0% Aloe vera increased the levels of mRNA for SOD and GPX1 respectively, but the levels of CAT were reduced in follicles cultured with 2.5%, 5.0%, 10.0% and 20.0%. Additionally, follicles cultured with 2.5% of Aloe vera had their ultrastructure well preserved, while those cultured with 5.0%, 10.0% and 20.0% exhibited increased oocyte vacuolization and damaged organelles. In conclusion, 2.5% Aloe vera increases antrum formation, viability and expression of mRNA for SOD in cultured secondary follicles, but higher concentrations of Aloe vera have negative effects on follicular ultrastructure. •Aloe vera (2.5%) increases antrum formation in cultured bovine secondary follicles.•Aloe vera (2.5% and 5.0%) improves in vitro secondary follicle viability rate.•Aloe vera (2.5% and 10.0%) increases the levels of SOD and GPX1 mRNA.•Follicles cultured with Aloe vera (2.5%) have well preserved ultrastructure.
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ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2022.107078