Search Results - "Paula S. Salgado"

Structure and assembly of the S-layer in C. difficile by Lanzoni-Mangutchi, Paola, Banerji, Oishik, Wilson, Jason, Barwinska-Sendra, Anna, Kirk, Joseph A., Vaz, Filipa, O’Beirne, Shauna, Baslé, Arnaud, El Omari, Kamel, Wagner, Armin, Fairweather, Neil F., Douce, Gillian R., Bullough, Per A., Fagan, Robert P., Salgado, Paula S.

“…Many bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface and plays crucial roles in cell physiology. Here,…”
Get full text

The engulfasome in C. difficile: Variations on protein machineries by Kelly, Abigail, Salgado, Paula S.

“…Clostridioides difficile infection (CDI) continues to be a substantial healthcare burden, and the changing disease profile raises new challenges in CDI…”
Get full text

Structure of the RNA-dependent RNA polymerase P2 from the cystovirus φ8 by Latimer-Smith, Merlyn, Salgado, Paula S., Forsyth, Ismay, Makeyev, Eugene, Poranen, Minna M., Stuart, Dave I., Grimes, Jonathan M., El Omari, Kamel

“…The replication of RNA viruses relies on the activity of RNA-dependent RNA polymerases (RdRps). Despite large variations in their genomic sequences, viral…”
Get full text

Peptidoglycan degradation machinery in Clostridium difficile forespore engulfment by Dembek, Marcin, Kelly, Abigail, Barwinska‐Sendra, Anna, Tarrant, Emma, Stanley, Will A., Vollmer, Daniela, Biboy, Jacob, Gray, Joe, Vollmer, Waldemar, Salgado, Paula S.

“…Summary Clostridium difficile remains the leading cause of antibiotic‐associated diarrhoea in hospitals worldwide, linked to significant morbidity and…”
Get full text

Structural basis for the broad specificity to host-cell ligands by the pathogenic fungus Candida albicans by Salgado, Paula S., Yan, Robert, Taylor, Jonathan D., Burchell, Lynn, Jones, Rhian, Hoyer, Lois L., Matthews, Steve J., Simpson, Peter J., Cota, Ernesto

“…Candida albicans is the most prevalent fungal pathogen in humans and a major source of life-threatening nosocomial infections. The Ais (a̲gglutinin-l̲ike…”
Get full text

Redox Regulation, Rather than Stress-Induced Phosphorylation, of a Hog1 Mitogen-Activated Protein Kinase Modulates Its Nitrosative-Stress-Specific Outputs by Herrero-de-Dios, Carmen, Day, Alison M, Tillmann, Anna T, Kastora, Stavroula L, Stead, David, Salgado, Paula S, Quinn, Janet, Brown, Alistair J P

“…In all eukaryotic kingdoms, mitogen-activated protein kinases (MAPKs) play critical roles in cellular responses to environmental cues. These MAPKs are…”
Get full text

Atomic Resolution Insights into Curli Fiber Biogenesis by Taylor, Jonathan D., Zhou, Yizhou, Salgado, Paula S., Patwardhan, Ardan, McGuffie, Matt, Pape, Tillmann, Grabe, Grzegorz, Ashman, Elisabeth, Constable, Sean C., Simpson, Peter J., Lee, Wei-chao, Cota, Ernesto, Chapman, Matthew R., Matthews, Steve J.

“…Bacteria produce functional amyloid fibers called curli in a controlled, noncytotoxic manner. These extracellular fimbriae enable biofilm formation and promote…”
Get full text

Inducible Expression of spo0A as a Universal Tool for Studying Sporulation in Clostridium difficile by Dembek, Marcin, Willing, Stephanie E, Hong, Huynh A, Hosseini, Siamand, Salgado, Paula S, Cutting, Simon M

“…remains a leading nosocomial pathogen, putting considerable strain on the healthcare system. The ability to form endospores, highly resistant to environmental…”
Get full text

Metabolic cofactors NADH and FAD act as non-canonical initiating substrates for a primase and affect replication primer processing in vitro by Julius, Christina, Salgado, Paula S, Yuzenkova, Yulia

“…Abstract To initiate replication on a double-stranded DNA de novo, all organisms require primase, an RNA polymerase making short RNA primers which are then…”
Get full text

The structure of an RNAi polymerase links RNA silencing and transcription by Salgado, Paula S, Koivunen, Minni R L, Makeyev, Eugene V, Bamford, Dennis H, Stuart, David I, Grimes, Jonathan M

“…RNA silencing refers to a group of RNA-induced gene-silencing mechanisms that developed early in the eukaryotic lineage, probably for defence against pathogens…”
Get full text

Extending the usability of the phasing power of diselenide bonds: SeCys SAD phasing of CsgC using a non-auxotrophic strain by Salgado, Paula S., Taylor, Jonathan D., Cota, Ernesto, Matthews, Steve J.

“…The CsgC protein is a component of the curli system in Escherichia coli. Reported here is the successful incorporation of selenocysteine (SeCys) and…”
Get full text

A practical overview of molecular replacement: Clostridioides difficile PilA1, a difficult case study by Crawshaw, Adam D., Baslé, Arnaud, Salgado, Paula S.

“…Many biologists are now routinely seeking to determine the three‐dimensional structures of their proteins of choice, illustrating the importance of this…”
Get full text

Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile by Martins, Diogo, Nerber, Hailee N., Roughton, Charlotte G., Fasquelle, Amaury, Barwinska‐Sendra, Anna, Vollmer, Daniela, Gray, Joe, Vollmer, Waldemar, Sorg, Joseph A., Salgado, Paula S., Henriques, Adriano O., Serrano, Mónica

“…In the model organism Bacillus subtilis, a signaling protease produced in the forespore, SpoIVB, is essential for the activation of the sigma factor σK, which…”
Get full text

The Peptide-binding Cavity Is Essential for Als3-mediated Adhesion of Candida albicans to Human Cells by Lin, Jing, Oh, Soon-Hwan, Jones, Rhian, Garnett, James A., Salgado, Paula S., Rusnakova, Sophia, Matthews, Steve J., Hoyer, Lois L., Cota, Ernesto

“…The adhesive phenotype of Candida albicans contributes to its ability to colonize the host and cause disease. Als proteins are one of the most widely studied…”
Get full text

The SpoIIQ‐SpoIIIAH complex of Clostridium difficile controls forespore engulfment and late stages of gene expression and spore morphogenesis by Serrano, Mónica, Crawshaw, Adam D., Dembek, Marcin, Monteiro, João M., Pereira, Fátima C., Pinho, Mariana Gomes, Fairweather, Neil F., Salgado, Paula S., Henriques, Adriano O.

“…Summary Engulfment of the forespore by the mother cell is a universal feature of endosporulation. In Bacillus subtilis, the forespore protein SpoIIQ and the…”
Get full text

An intact S-layer is advantageous to Clostridioides difficile within the host by Ormsby, Michael J, Vaz, Filipa, Kirk, Joseph A, Barwinska-Sendra, Anna, Hallam, Jennifer C, Lanzoni-Mangutchi, Paola, Cole, John, Chaudhuri, Roy R, Salgado, Paula S, Fagan, Robert P, Douce, Gillian R

“…Clostridioides difficile is responsible for substantial morbidity and mortality in antibiotically-treated, hospitalised, elderly patients, in which toxin…”
Get full text

A mother cell-to-forespore channel: current understanding and future challenges by Crawshaw, Adam D., Serrano, Mónica, Stanley, Will A., Henriques, Adriano O., Salgado, Paula S.

“…Abstract Formation of endospores allows some bacteria to survive extreme nutrient limitation. The resulting dormant cell, the spore, persists in the…”
Get full text

In vacuo X-ray data collection from graphene-wrapped protein crystals by Warren, Anna J., Crawshaw, Adam D., Trincao, Jose, Aller, Pierre, Alcock, Simon, Nistea, Ioana, Salgado, Paula S., Evans, Gwyndaf

“…The measurement of diffraction data from macromolecular crystal samples held in vacuo holds the promise of a very low X‐ray background and zero absorption of…”
Get full text

Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure by SANTORO, Giani F, DAS GRACAS CARDOSO, Maria, GUIMARÄES, Luiz Gustavo L, SALGADO, Ana Paula S. P, MENNA-BARRETO, Rubem F. S, SOARES, Maurilio J

“…In the present work, we have investigated the effect of essential oils obtained from Origanum vulgare L. (oregano) and Thymus vulgaris L. (thyme) on growth and…”
Get full text

Regulation of the Plasmodium motor complex: phosphorylation of myosin A tail-interacting protein (MTIP) loosens its grip on MyoA by Douse, Christopher H, Green, Judith L, Salgado, Paula S, Simpson, Peter J, Thomas, Jemima C, Langsley, Gordon, Holder, Anthony A, Tate, Edward W, Cota, Ernesto

“…The interaction between the C-terminal tail of myosin A (MyoA) and its light chain, myosin A tail domain interacting protein (MTIP), is an essential feature of…”
Get full text