Evaluation of Etest and macrodilution broth method for antifungal susceptibility testing of Candida sp strains isolated from oral cavities of AIDS patients

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer&#...

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Bibliographic Details
Published in:	Revista do Instituto de Medicina Tropical de São Paulo Vol. 44; no. 3; pp. 121 - 125
Main Authors:	Silva, Maria do Rosário R, Costa, Márcio R, Miranda, André T B, Fernandes, Orionalda de F L, Costa, Carolina R, Paula, Claudete R de
Format:	Journal Article
Language:	English
Published:	Brazil Instituto de Medicina Tropical de Sao Paulo 2002 Instituto de Medicina Tropical Universidade de São Paulo (USP)
Subjects:	Antifungal Agents - pharmacology Antifungal susceptibility tests Broth macrodilution Candida - drug effects Culture Media Etest Fluconazole - pharmacology Humans Itraconazole - pharmacology Microbial Sensitivity Tests - methods TROPICAL MEDICINE Broth macrodilution Etest Antifungal susceptibility tests
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Summary:	A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at +/- 2 dilutions for both methods were considered, the agreement rates were 90.4% for itraconazole, ketoconazole and amphotericin B and 84.6% for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0036-4665 1678-9946 0036-4665 1678-9946
DOI:	10.1590/S0036-46652002000300002