Search Results - "Patten, Phillip A."

Activity-Based DNA-Encoded Library Screening for Selective Inhibitors of Eukaryotic Translation by Barhoosh, Huda, Dixit, Anjali, Cochrane, Wesley G., Cavett, Valerie, Prince, Robin N., Blair, Brooke O., Ward, Fred R., McClure, Kim F., Patten, Phillip A., Paulick, Margot G., Paegel, Brian M.

“…Small molecule probes exist for only ∼2% of human proteins because most lack functional binding pockets or cannot be assayed for high-throughput screening…”
Get full text

Discovery and directed evolution of a glyphosate tolerance gene by Castle, L.A, Siehl, D.L, Gorton, R, Patten, P.A, Chen, Y.H, Bertain, S, Cho, H.J, Duck, N, Wong, J, Liu, D

“…The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting…”
Get full text

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins by Kurtzman, Aaron L, Govindarajan, Sridhar, Vahle, Katherine, Jones, Jennifer T, Heinrichs, Volker, Patten, Phillip A

“…Recent developments in directed evolution technologies combined with innovations in robotics and screening methods have revolutionized protein engineering…”
Get full text

Applications of DNA shuffling to pharmaceuticals and vaccines by Patten, Phillip A, Howard, Russell J, Stemmer, Willem PC

“…DNA shuffling is a practical process for directed molecular evolution which uses recombination to dramatically accelerate the rate at which one can evolve…”
Get full text

The Immunological Evolution of Catalysis by Patten, Phillip A., Gray, Nathanael S., Yang, Priscilla L., Marks, Cara B., Wedemayer, Gary J., Boniface, J. Jay, Stevens, Raymond C., Schultz, Peter G.

“…The germline genes used by the mouse to generate the esterolytic antibody 48G7 were cloned and expressed in an effort to increase our understanding of the…”
Get full text

Structural Insights into the Evolution of an Antibody Combining Site by Wedemayer, Gary J., Patten, Phillip A., Wang, Leo H., Schultz, Peter G., Stevens, Raymond C.

“…The crystal structures of a germline antibody Fab fragment and its complex with hapten have been solved at 2.1 Å resolution. These structures are compared with…”
Get full text

Evolution of a cytokine using DNA family shuffling by Patten, Phillip A, Chang, Chia-Chun J, Chen, Teddy T, Cox, Brett W, Dawes, Glenn N, Stemmer, Willem P.C, Punnonen, Juha

“…DNA shuffling of a family of over 20 human interferon-alpha (Hu-IFN-alpha) genes was used to derive variants with increased antiviral and antiproliferation…”
Get full text

Directed evolution of gene-shuffled IFN-α molecules with activity profiles tailored for treatment of chronic viral diseases by Brideau-Andersen, Amy D, Huang, Xiaojian, Sun, Siu-Chi Chang, Chen, Teddy T, Stark, Diane, Sas, Ian J, Zadik, Linda, Dawes, Glenn N, Guptill, Douglas R, McCord, Robert, Govindarajan, Sridhar, Roy, Ajoy, Yang, Shumin, Gao, Judy, Chen, Yong Hong, Skartved, Niels Jørgen Ø, Pedersen, Annette K, Lin, David, Locher, Christopher P, Rebbapragada, Indrani, Jensen, Anne Dam, Bass, Steven H, Nissen, Torben L. Straight, Viswanathan, Sridhar, Foster, Graham R, Symons, Julian A, Patten, Phillip A

“…Type I IFNs are unusually pleiotropic cytokines that bind to a single heterodimeric receptor and have potent antiviral, antiproliferative, and immune…”
Get full text

The adult T-cell receptor 5-chain is diverse and distinct from that of fetal thymocytes by Francis Elliott, John, Rock, Edwin P, Patten, Phillip A, Davis, Mark M, Chien, Yueh-hsiu

Get full text

New and modified interferon alfas: preclinical and clinical data by Masci, Paul, Bukowski, Ronald M, Patten, Phillip A, Osborn, Blaire L, Borden, Ernest C

“…Recombinant human interferon (IFN)-alpha was the first biotherapeutic agent approved by the US Food and Drug Administration for the treatment of a human…”
Get full text

A Genetic Approach to the Generation of Antibodies with Enhanced Catalytic Activities by Lesley, Scott A., Patten, Phillip A., Schultz, Peter G.

“…A hydrolytic catalytic antibody, generated against a nitrophenyl phosphonate transition state analogue, has been cloned and expressed in Escherichia coli for…”
Get full text

Crystal structures of the free and liganded form of an esterolytic catalytic antibody 1 1Edited by I. A. Wilson by Wedemayer, Gary J, Wang, Leo H, Patten, Phillip A, Schultz, Peter G, Stevens, Raymond C

Get full text

Crystal structures of the free and liganded form of an esterolytic catalytic antibody by Wedemayer, G J, Wang, L H, Patten, P A, Schultz, P G, Stevens, R C

“…The crystal structure of the esterase catalytic antibody 48G7 has been determined in the presence of hapten at 2.0 A resolution and in the absence of hapten at…”
Get full text

Transfer of putative complementarity-determining region loops of T cell receptor V domains confers toxin reactivity but not peptide/MHC specificity by Patten, PA, Rock, EP, Sonoda, T, Fazekas de St. Groth, B, Jorgensen, JL, Davis, MM

“…We have used multiple-amino acid replacement mutagenesis to examine the roles of the TCR homologues of Ig complementarity-determining regions (CDR) and…”
Get full text

Directed evolution studies with combinatorial libraries of T4 lysozyme mutants by Patten, P A, Sonoda, T, Davis, M M

“…Gene duplication with divergence to new functions has been an important mechanism in protein evolution. However, the questions of how many new functions can…”
Get full text

The Adult T-Cell Receptor (Gamma)-Chain Is Diverse and Distinct from That of Fetal Thymocytes by Elliott, John Francis, Rock, Edwin P, Patten, Phillip A, Davis, Mark M, Chien, Yueh-hsiu

“…T lymphocytes recognize foreign molecules using the T-cell receptor (TCR), a disulphide-linked heterodimer closely associated with the CD3 polypeptide complex…”
Get full text