Rhizoferrin Glycosylation in Rhizopus microsporus

Rhizoferrin Glycosylation in Rhizopus microsporus

Rhizopus spp. are the most common etiological agents of mucormycosis, causing over 90% mortality in disseminated infections. The diagnosis relies on histopathology, culture, and/or polymerase chain reaction. For the first time, the glycosylation of rhizoferrin (RHF) was described in a Rhizopus micro...

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Bibliographic Details
Published in:Journal of fungi (Basel) Vol. 6; no. 2; p. 89
Main Authors: Skriba, Anton, Patil, Rutuja Hiraji, Hubacek, Petr, Dobias, Radim, Palyzova, Andrea, Maresova, Helena, Pluhacek, Tomas, Havlicek, Vladimir
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-06-2020
MDPI
Subjects:
Biotransformation
Chromatography
Communication
Deoxyribonucleic acid
DNA
Fermentation
Fungi
glycoside
Glycosylation
Infections
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical research
metabolite
Metabolites
Mucormycosis
Polymerase chain reaction
Posaconazole
rhizoferrin
Rhizopus microsporus
Scientific imaging
siderophore
Software
Virulence
United States
Czech Republic
United States > US
Germany
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Summary:Rhizopus spp. are the most common etiological agents of mucormycosis, causing over 90% mortality in disseminated infections. The diagnosis relies on histopathology, culture, and/or polymerase chain reaction. For the first time, the glycosylation of rhizoferrin (RHF) was described in a Rhizopus microsporus clinical isolate by liquid chromatography and accurate tandem mass spectrometry. The fermentation broth lyophilizate contained 345.3 ± 13.5, 1.2 ± 0.03, and 0.03 ± 0.002 mg/g of RHF, imido-RHF, and bis-imido-RHF, respectively. Despite a considerable RHF secretion rate, we did not obtain conclusive RHF detection from a patient with disseminated mucormycosis caused by the same R. microsporus strain. We hypothesize that parallel antimycotic therapy, RHF biotransformation, and metabolism compromised the analysis. On the other hand, the full profile of posaconazole metabolites was retrieved by our in house software CycloBranch.
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ISSN:2309-608X
2309-608X
DOI:10.3390/jof6020089