Search Results - "Pathak, Gopal P."

Optimized second-generation CRY2–CIB dimerizers and photoactivatable Cre recombinase by Taslimi, Amir, Zoltowski, Brian, Miranda, Jose G, Pathak, Gopal P, Hughes, Robert M, Tucker, Chandra L

“…Identification of minimal functional CRY2–CIB1 domains and mutations that increased CRY2 photocycle lifetimes combined with the development of an improved…”
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Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2 by Pathak, Gopal P, Spiltoir, Jessica I, Höglund, Camilla, Polstein, Lauren R, Heine-Koskinen, Sari, Gersbach, Charles A, Rossi, Jari, Tucker, Chandra L

“…Optogenetic tools allow regulation of cellular processes with light, which can be delivered with spatiotemporal resolution. In previous work, we used…”
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Temporal Effect on PD‐L1 Detection and Novel Insights Into Its Clinical Implications in Non–Small Cell Lung Cancer by Pathak, Gopal P., Shah, Rashmi, Castonguay, Mathieu, Cheng, Angela, Fris, John, Murphy, Rowan, Darling, Gail, Ednie, Alexander, French, Daniel, Henteleff, Harry, Mujoomdar, Aneil, Plourde, Madelaine, Wallace, Alison, Xu, Zhaolin

“…ABSTRACT Objectives Several studies rely on archived tissue blocks to assess the PD‐L1 scores; however, a detailed analysis of potential variations of scores…”
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Optogenetic control of cell function using engineered photoreceptors by Pathak, Gopal P., Vrana, Justin D., Tucker, Chandra L.

“…Over the past decades, there has been growing recognition that light can provide a powerful stimulus for biological interrogation. Light‐actuated tools allow…”
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The NAD+ Salvage Pathway Supports PHGDH-Driven Serine Biosynthesis by Murphy, J. Patrick, Giacomantonio, Michael A., Paulo, Joao A., Everley, Robert A., Kennedy, Barry E., Pathak, Gopal P., Clements, Derek R., Kim, Youra, Dai, Cathleen, Sharif, Tanveer, Gygi, Steven P., Gujar, Shashi

“…NAD+ is a key metabolic redox cofactor that is regenerated from nicotinamide through the NAD+ salvage pathway. Here, we find that inhibiting the NAD+ salvage…”
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LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves by Moriconi, Victoria, Sellaro, Romina, Ayub, Nicolás, Soto, Gabriela, Rugnone, Matías, Shah, Rashmi, P. Pathak, Gopal, Gärtner, Wolfgang, Casal, Jorge J.

“…Summary In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated…”
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NAD+ depletion enhances reovirus-induced oncolysis in multiple myeloma by Kennedy, Barry E., Giacomantonio, Michael, Murphy, J. Patrick, Cutler, Samuel, Sadek, Maryanne, Konda, Prathyusha, Paulo, Joao A., Pathak, Gopal P., Renkens, Saskia H.J., Grieve, Stacy, Pol, Jonathan, Gygi, Steven P., Richardson, Christopher, Gaston, Daniel, Reiman, Anthony, Kroemer, Guido, Elnenaei, Manal O., Gujar, Shashi A.

“…Cancer cell energy metabolism plays an important role in dictating the efficacy of oncolysis by oncolytic viruses. To understand the role of multiple myeloma…”
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RTN4 Knockdown Dysregulates the AKT Pathway, Destabilizes the Cytoskeleton, and Enhances Paclitaxel-Induced Cytotoxicity in Cancers by Pathak, Gopal P., Shah, Rashmi, Kennedy, Barry E., Murphy, J. Patrick, Clements, Derek, Konda, Prathyusha, Giacomantonio, Michael, Xu, Zhaolin, Schlaepfer, Isabel R., Gujar, Shashi

“…[Display omitted] Reticulon-4 (RTN4), commonly known as a neurite outgrowth inhibitor (Nogo), is emerging as an important player in human cancers. Clinically,…”
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Inhibition of Pyruvate Dehydrogenase Kinase Enhances the Antitumor Efficacy of Oncolytic Reovirus by Kennedy, Barry E, Murphy, John Patrick, Clements, Derek R, Konda, Prathyusha, Holay, Namit, Kim, Youra, Pathak, Gopal P, Giacomantonio, Michael A, Hiani, Yassine El, Gujar, Shashi

“…Oncolytic viruses (OV) such as reovirus preferentially infect and kill cancer cells. Thus, the mechanisms that dictate the susceptibility of cancer cells to…”
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Benchmarking of Optical Dimerizer Systems by Pathak, Gopal P, Strickland, Devin, Vrana, Justin D, Tucker, Chandra L

“…Optical dimerizers are a powerful new class of optogenetic tools that allow light-inducible control of protein–protein interactions. Such tools have been…”
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Metagenome-based Screening Reveals Worldwide Distribution of LOV-Domain Proteins by Pathak, Gopal P., Losi, Aba, Gärtner, Wolfgang

“…Metagenomes from various environments were screened for sequences homologous to light, oxygen, voltage (LOV)‐domain proteins. LOV domains are flavin binding,…”
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Extremophilic Acinetobacter Strains from High-Altitude Lakes in Argentinean Puna: Remarkable UV-B Resistance and Efficient DNA Damage Repair by Albarracín, Virginia Helena, Pathak, Gopal P., Douki, Thierry, Cadet, Jean, Borsarelli, Claudio Darío, Gärtner, Wolfgang, Farias, María Eugenia

“…High-Altitude Andean Lakes (HAAL) of the South American Andes are almost unexplored ecosystems of shallow lakes. The HAAL are recognized by a remarkably high…”
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First characterisation of a CPD-class I photolyase from a UV-resistant extremophile isolated from High-Altitude Andean Lakes by Albarracín, Virginia Helena, Simon, Julian, Pathak, Gopal P, Valle, Lorena, Douki, Thierry, Cadet, Jean, Borsarelli, Claudio Darío, Farias, María Eugenia, Gärtner, Wolfgang

“…UV-resistant Acinetobacter sp. Ver3 isolated from High-Altitude Andean Lakes (HAAL) in Argentinean Puna, one of the highest UV exposed ecosystems on Earth,…”
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Detection and isolation of selected genes of interest from metagenomic libraries by a DNA microarray approach by Pathak, Gopal P, Gärtner, Wolfgang

“…A DNA microarray-based approach is described for screening metagenomic libraries for the presence of selected genes. The protocol is exemplified for the…”
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LOV ‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of P seudomonas syringae in light‐exposed A rabidopsis leaves by Moriconi, Victoria, Sellaro, Romina, Ayub, Nicolás, Soto, Gabriela, Rugnone, Matías, Shah, Rashmi, P. Pathak, Gopal, Gärtner, Wolfgang, Casal, Jorge J.

“…In A rabidopsis thaliana , light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component…”
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