Search Results - "Pater, Sylvia"

Profiling Cas9‐ and Cas12a‐induced mutagenesis in Arabidopsis thaliana by Pater, Sylvia, Kamoen, Lycka, Schendel, Robin, Hooykaas, Paul J. J., Tijsterman, Marcel

“…SUMMARY With the advancement of CRISPR technologies, a comprehensive understanding of repair mechanisms following double‐strand break (DSB) formation is…”
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Gene targeting in polymerase theta‐deficient Arabidopsis thaliana by Tol, Niels, Schendel, Robin, Bos, Alex, Kregten, Maartje, Pater, Sylvia, Hooykaas, Paul J.J., Tijsterman, Marcel

“…SUMMARY Agrobacterium tumefaciens‐mediated transformation has been for decades the preferred tool to generate transgenic plants. During this process, a T‐DNA…”
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ZFN-mediated gene targeting of the Arabidopsis protoporphyrinogen oxidase gene through Agrobacterium-mediated floral dip transformation by de Pater, Sylvia, Pinas, Johan E, Hooykaas, Paul J J, van der Zaal, Bert J

“…Previously, we showed that ZFN-mediated induction of double-strand breaks (DSBs) at the intended recombination site enhanced the frequency of gene targeting…”
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Distinct mechanisms for genomic attachment of the 5′ and 3′ ends of Agrobacterium T-DNA in plants by Kralemann, Lejon E. M., de Pater, Sylvia, Shen, Hexi, Kloet, Susan L., van Schendel, Robin, Hooykaas, Paul J. J., Tijsterman, Marcel

“…Agrobacterium tumefaciens , a pathogenic bacterium capable of transforming plants through horizontal gene transfer, is nowadays the preferred vector for plant…”
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The SLEEPER genes: a transposase-derived angiosperm-specific gene family by Knip, Marijn, de Pater, Sylvia, Hooykaas, Paul J J

“…DAYSLEEPER encodes a domesticated transposase from the hAT-superfamily, which is essential for development in Arabidopsis thaliana. Little is known about the…”
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CRISPR/Cas9-Induced Double-Strand Break Repair in Arabidopsis Nonhomologous End-Joining Mutants by Shen, Hexi, Strunks, Gary D, Klemann, Bart J P M, Hooykaas, Paul J J, de Pater, Sylvia

“…Double-strand breaks (DSBs) are one of the most harmful DNA lesions. Cells utilize two main pathways for DSB repair: homologous recombination (HR) and…”
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ZFN-induced mutagenesis and gene-targeting in Arabidopsis through Agrobacterium-mediated floral dip transformation by de Pater, Sylvia, Neuteboom, Leon W, Pinas, Johan E, Hooykaas, Paul J J, van der Zaal, Bert J

“…Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs…”
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Epigenetic alterations at genomic loci modified by gene targeting in Arabidopsis thaliana by Lieberman-Lazarovich, Michal, Melamed-Bessudo, Cathy, de Pater, Sylvia, Levy, Avraham A

“…Gene Targeting (GT) is the integration of an introduced vector into a specific chromosomal site, via homologous recombination. It is considered an effective…”
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Manipulation of starch granule size distribution in potato tubers by modulation of plastid division by de Pater, Sylvia, Caspers, Martien, Kottenhagen, Marijke, Meima, Henk, ter Stege, Renaldo, de Vetten, Nick

“…Starch granule size is an important parameter for starch applications in industry. Starch granules are formed in amyloplasts, which are, like chloroplasts,…”
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B‐type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression by Langeveld, Sandra M.J., van Wijk, Ringo, Stuurman, Nico, Kijne, Jan W., de Pater, Sylvia

“…Starch granules in mature wheat endosperm show a bimodal size distribution. The formation of small starch granules in wheat endosperm cells was studied by…”
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Glucosylation Activity and Complex Formation of Two Classes of Reversibly Glycosylated Polypeptides by Sandra M. J. Langeveld, Marco Vennik, Marijke Kottenhagen, Ringo van Wijk, Ankie Buijk, Kijne, Jan W., de Pater, Sylvia

“…Reversibly glycosylated polypeptides (RGPs) have been implicated in polysaccharide biosynthesis. In plants, these proteins may function, for example, in cell…”
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T-DNA integration in plants results from polymerase-θ-mediated DNA repair by van Kregten, Maartje, de Pater, Sylvia, Romeijn, Ron, van Schendel, Robin, Hooykaas, Paul J. J., Tijsterman, Marcel

“…Agrobacterium tumefaciens is a pathogenic bacterium, which transforms plants by transferring a discrete segment of its DNA, the T-DNA, to plant cells. The…”
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Programmed Cell Death in the Leaves of the Arabidopsis Spontaneous Necrotic Spots (sns-D) Mutant Correlates with Increased Expression of the Eukaryotic Translation Initiation Factor eIF4B2 by Gaussand, Gwénaël M D J-M, Jia, Qi, van der Graaff, Eric, Lamers, Gerda E M, Fransz, Paul F, Hooykaas, Paul J J, de Pater, Sylvia

“…From a pool of transgenic Arabidopsis (Arabidopsis thaliana) plants harboring an activator T-DNA construct, one mutant was identified that developed…”
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True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template by de Pater, Sylvia, Klemann, Bart J. P. M., Hooykaas, Paul J. J.

“…In recent years, several tools have become available for improved gene-targeting (GT) in plants. DNA breaks at specific sites activate local DNA repair and…”
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bZIP proteins bind to a palindromic sequence without an ACGT core located in a seed‐specific element of the pea lectin promoter by Pater, Sylvia, Katagiri, Fumiaki, Kijne, Jan, Chua, Nam‐Hai

“…Summary Previously, it has been shown that a trimer of a 22 bp fragment of the promoter of the seed‐specific pea lectin gene confers high expression in seed…”
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Poly(ADP-ribose)polymerases are involved in microhomology mediated back-up non-homologous end joining in Arabidopsis thaliana by Jia, Qi, Dulk-Ras, Amke den, Shen, Hexi, Hooykaas, Paul J. J., de Pater, Sylvia

“…Besides the KU-dependent classical non-homologous end-joining (C-NHEJ) pathway, an alternative NHEJ pathway first identified in mammalian systems, which is…”
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Genetic dissection of mutagenic repair and T-DNA capture at CRISPR-induced DNA breaks in Arabidopsis thaliana by Kamoen, Lycka, Kralemann, Lejon E M, van Schendel, Robin, van Tol, Niels, Hooykaas, Paul J J, de Pater, Sylvia, Tijsterman, Marcel

“…A practical and powerful approach for genome editing in plants is delivery of CRISPR reagents via transformation. The double-strand break (DSB)-inducing enzyme…”
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DAYSLEEPER: a nuclear and vesicular-localized protein that is expressed in proliferating tissues by Knip, Marijn, Hiemstra, Steven, Sietsma, Afke, Castelein, Marina, de Pater, Sylvia, Hooykaas, Paul

“…DAYSLEEPER is a domesticated transposase that is essential for development in Arabidopsis thaliana [Nature, 436:282-284, 2005]. It is derived from a…”
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CRISPR/Cas9 Mutagenesis by Translocation of Cas9 Protein Into Plant Cells via the Agrobacterium Type IV Secretion System by Schmitz, Daan J., Ali, Zahir, Wang, Chenglong, Aljedaani, Fatimah, Hooykaas, Paul J. J., Mahfouz, Magdy, de Pater, Sylvia

“…Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a powerful tool for genome engineering in plants. The…”
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The SLEEPERgenes: a transposase-derived angiosperm-specific gene family by Knip, Marijn, de Pater, Sylvia, Hooykaas, Paul JJ

“…DAYSLEEPER encodes a domesticated transposase from the hAT-superfamily, which is essential for development in Arabidopsis thaliana. Little is known about the…”
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