Synthesis of Dihydroquinoline-Based Derivatives of Fluorescent Rhodamine Dyes for Nucleic Acid Analysis by a Real-Time Polymerase Chain Reaction

Synthesis of Dihydroquinoline-Based Derivatives of Fluorescent Rhodamine Dyes for Nucleic Acid Analysis by a Real-Time Polymerase Chain Reaction

An optimized synthesis scheme for the dihydroquinoline-based derivatives of xanthene fluorescent dye has been developed. For the first time, their use as effective fluorophores in hybridization probes for real-time PCR has been demonstrated with the detection on an individual spectral channel.

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry Vol. 46; no. 3; pp. 349 - 359
Main Authors: Matvienko, I. V., Bayramov, V. M., Parygina, N. A., Kurochkin, V. E., Alekseev, Y. I.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-05-2020
Springer Nature B.V
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Chemical compounds
Chemical synthesis
Derivatives
Dyes
Fluorescence
Fluorescent dyes
Life Sciences
Organic Chemistry
Polymerase chain reaction
Real time
Rhodamine
quinolones
nucleic acids
fluorescent rhodamine dyes
real-time polymerase chain reaction
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Description
Summary:An optimized synthesis scheme for the dihydroquinoline-based derivatives of xanthene fluorescent dye has been developed. For the first time, their use as effective fluorophores in hybridization probes for real-time PCR has been demonstrated with the detection on an individual spectral channel.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162020030127