Placental expression of imprinted genes varies with sampling site and mode of delivery

Abstract Imprinted genes, which are monoallelically expressed by virtue of an epigenetic process initiated in the germline, are known to play key roles in regulating fetal growth and placental development. Numerous studies are investigating the expression of these imprinted genes in the human placen...

Full description

Saved in:

Bibliographic Details
Published in:	Placenta (Eastbourne) Vol. 36; no. 8; pp. 790 - 795
Main Authors:	Janssen, A.B, Tunster, S.J, Savory, N, Holmes, A, Beasley, J, Parveen, S.A.R, Penketh, R.J.A, John, R.M
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 01-08-2015 W.B. Saunders
Subjects:	Adult Apoptosis Regulatory Proteins Cyclin-Dependent Kinase Inhibitor p57 - genetics Cyclin-Dependent Kinase Inhibitor p57 - metabolism Delivery mode Delivery, Obstetric - methods DNA-Binding Proteins Female Gene Expression Regulation, Developmental Genomic Imprinting Gestational Age Humans Imprinted genes Internal Medicine Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism Male Nuclear Proteins - genetics Nuclear Proteins - metabolism Obstetrics and Gynecology Placenta Placenta - metabolism Pregnancy Proteins - genetics Proteins - metabolism RNA-Binding Proteins Sampling Sex Factors Young Adult Delivery mode Placenta Sampling Imprinted genes
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Abstract Imprinted genes, which are monoallelically expressed by virtue of an epigenetic process initiated in the germline, are known to play key roles in regulating fetal growth and placental development. Numerous studies are investigating the expression of these imprinted genes in the human placenta in relation to common complications of pregnancy such as fetal growth restriction and preeclampsia. This study aimed to determine whether placental sampling protocols or other factors such as fetal sex, gestational age and mode of delivery may influence the expression of imprinted genes predicted to regulate placental signalling. Methods Term placentas were collected from Caucasian women delivering at University Hospital of Wales or Royal Gwent Hospital within two hours of delivery. Expression of the imprinted genes PHLDA2 , CDKN1C , PEG3 and PEG10 was assayed by quantitative real time PCR. Intraplacental gene expression was analysed (N = 5). Placental gene expression was compared between male (N = 11) and female (N = 11) infants, early term (N = 8) and late term (N = 10) deliveries and between labouring (N = 13) and non-labouring (N = 21) participants. Results The paternally expressed imprinted genes PEG3 and PEG10 were resilient to differences in sampling site, fetal sex, term gestational age and mode of delivery. The maternally expressed imprinted gene CDKN1C was elevated over 2-fold (p < 0.001) in placenta from labouring deliveries compared with elective caesarean sections. In addition, the maternally expressed imprinted gene PHLDA2 was elevated by 1.8 fold (p = 0.01) in samples taken at the distal edge of the placenta compared to the cord insertion site. Conclusion These findings support the reinterpretation of existing data sets on these genes in relation to complications of pregnancy and further reinforce the importance of optimising and unifying placental collection protocols for future studies.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0143-4004 1532-3102
DOI:	10.1016/j.placenta.2015.06.011