Exploiting heterozygosity in industrial yeasts to create new and improved baker's yeasts

Exploiting heterozygosity in industrial yeasts to create new and improved baker's yeasts

The main aim of the work was to utilize heterozygosity of industrial yeast strains to construct new baker's yeast strains. Commercial baker's yeast strain ALKO 743, its more ethanol tolerant descendant ALKO 554 selected initially for growth over 300 generations in increasing ethanol concen...

Full description

Saved in:
Bibliographic Details
Published in:Yeast (Chichester, England) Vol. 36; no. 9; pp. 571 - 587
Main Authors: Korhola, Matti, Naumova, Elena S., Partti, Edvard, Aittamaa, Marja, Turakainen, Hilkka, Naumov, Gennadi I.
Format: Journal Article
Language:English
Published: England Wiley Subscription Services, Inc 01-09-2019
Subjects:
baking
Ethanol
ethanol tolerance
Fermentation
genetical analysis
Heterosis
Heterozygosity
Hybrids
industrial
Meiosis
Ploidy
Saccharomyces cerevisiae
Sourdough
Sporulation
Sugar
Yeast
heterozygosity
baking
industrial
heterosis
ethanol tolerance
Saccharomyces cerevisiae
genetical analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The main aim of the work was to utilize heterozygosity of industrial yeast strains to construct new baker's yeast strains. Commercial baker's yeast strain ALKO 743, its more ethanol tolerant descendant ALKO 554 selected initially for growth over 300 generations in increasing ethanol concentrations in a glucose medium, and ALKO 3460 from an old domestic sour dough starter were used as starting strains. Isolated meiotic segregants of the strains were characterized genetically for sporulation ability and mating type, and the ploidy was determined physically. Heterozygosity of the segregant strains was estimated by a variety of molecular characterizations and fermentation and growth assays. The results showed wide heterozygosity and that the segregants were clustered into subgroups. This clustering was used for choosing distantly or closely related partners for strain construction crosses. Intrastrain hybrids made with segregants of ALKO 743 showed 16–24% hybrid vigour or heterosis. Interstrain hybrids with segregants of ALKO 743 and ALKO 3460 showed a wide variety of characteristics but also clear heterosis of 27–31% effects as assayed by lean and sugar dough raising. Distiller's yeast ALKO 554 turned out to be a diploid genetic segregant and not just a more ethanol tolerant mutant of the tetraploid parent strain ALKO 743.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.3428