Using NMR Spectroscopy To Measure Protein Binding Capacity on Gold Nanoparticles

Using NMR Spectroscopy To Measure Protein Binding Capacity on Gold Nanoparticles

A simple one-dimensional 1H NMR experiment that quantifies protein bound to gold nanoparticles has been developed for upper-division biochemistry and physical chemistry students. This laboratory experiment teaches the basics of NMR techniques, which is a highly effective tool in protein studies and...

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Bibliographic Details
Published in:Journal of chemical education Vol. 97; no. 3; pp. 820 - 824
Main Authors: Perera, Y. Randika, South, Taylor M, Hughes, Alex C, Parkhurst, Ashlyn N, Williams, Olivia C, Davidson, Mackenzie B, Wilks, Chloe A, Mlsna, Debra A, Fitzkee, Nicholas C
Format: Journal Article
Language:English
Published: United States American Chemical Society and Division of Chemical Education, Inc 10-03-2020
Division of Chemical Education, Inc
American Chemical Society
Subjects:
Binding
Biochemistry
Biosensors
Chemistry
Cytology
Diagnostic Tests
Drug delivery systems
Drug Therapy
Electronic Equipment
Gold
Laboratory Experiments
Macromolecules
Measurement
Molecular Structure
Molecules
Nanoparticles
NMR spectroscopy
Organic Chemistry
Physical chemistry
Proteins
Science Instruction
Scientific Concepts
Spectroscopy
Spectrum analysis
Students
Teaching Methods
Hands-On Learning/Manipulatives
NMR Spectroscopy
Upper-Division Undergraduate
Laboratory Instruction
Proteins/Peptides
Biophysical Chemistry
Biochemistry
Nanotechnology
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Summary:A simple one-dimensional 1H NMR experiment that quantifies protein bound to gold nanoparticles has been developed for upper-division biochemistry and physical chemistry students. This laboratory experiment teaches the basics of NMR techniques, which is a highly effective tool in protein studies and supports students to understand the concepts of NMR spectroscopy and nanoparticle–protein interactions. Understanding the interactions of gold nanoparticles (AuNPs) with biological macromolecules is becoming increasingly important as interest in the clinical use of nanoparticles has been on the rise. Applications in drug delivery, biosensing, diagnostics, and enhanced imaging are all tangible possibilities with a better understanding of AuNP–protein interactions. The ability to use AuNPs as biosensors for drug delivery methods in cellular uptake is dependent on the amount of protein that is able to bind to the surface of the nanoparticle. This laboratory experiment solidifies concepts such as quantitative NMR spectroscopy while reinforcing precision laboratory titrations. Students learn how 1H proton NMR spectra can be used to measure free protein in solution and protein bound to AuNPs. A simple formula is used to determine the binding capacity of the nanoparticle. This analysis helps students to understand the impact of nanoparticle–protein interactions, and it allows them to conceptualize macromolecular binding using NMR spectroscopy.
ISSN:0021-9584
1938-1328
DOI:10.1021/acs.jchemed.9b00625