Search Results - "Parker, Jared B."

Efficient engineering of human and mouse primary cells using peptide-assisted genome editing by Zhang, Zhen, Baxter, Amy E., Ren, Diqiu, Qin, Kunhua, Chen, Zeyu, Collins, Sierra M., Huang, Hua, Komar, Chad A., Bailer, Peter F., Parker, Jared B., Blobel, Gerd A., Kohli, Rahul M., Wherry, E. John, Berger, Shelley L., Shi, Junwei

“…Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary cells remains a major challenge. Here we describe an engineered…”
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Dynamics of Uracil and 5-Fluorouracil in DNA by Parker, Jared B, Stivers, James T

“…The prodrug 5-fluorouracil (5-FU), after activation into 5-F-dUMP, is an extensively used anticancer agent that inhibits thymidylate synthase and leads to…”
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Action and Timing of BacC and BacD in the Late Stages of Biosynthesis of the Dipeptide Antibiotic Bacilysin by Parker, Jared B, Walsh, Christopher T

“…Biosynthesis of the dipeptide antibiotic bacilysin, encoded by the seven Bacillus subtilis genes bacA–G, involves diversion of flux from prephenate to the…”
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Enzymatic capture of an extrahelical thymine in the search for uracil in DNA by Stivers, James T, Parker, Jared B, Bianchet, Mario A, Krosky, Daniel J, Friedman, Joshua I, Amzel, L. Mario

“…The enzyme uracil DNA glycosylase (UNG) excises unwanted uracil bases in the genome using an extrahelical base recognition mechanism. Efficient removal of…”
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Effect of the Thymidylate Synthase Inhibitors on dUTP and TTP Pool Levels and the Activities of DNA Repair Glycosylases on Uracil and 5-Fluorouracil in DNA by Grogan, Breeana C, Parker, Jared B, Guminski, Amy F, Stivers, James T

“…5-Fluorouracil (5-FU), 5-fluorodeoxyuridine (5-dUrd), and raltitrixed (RTX) are anticancer agents that target thymidylate synthase (TS), thereby blocking the…”
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Impact of linker strain and flexibility in the design of a fragment-based inhibitor by Stivers, James T, Chung, Suhman, Parker, Jared B, Bianchet, Mario, Amzel, L Mario

“…The linking together of molecular fragments that bind to adjacent sites on an enzyme can lead to high-affinity inhibitors. Ideally, this strategy would use…”
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Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors by Berríos, Kiara N, Barka, Aleksia, Gill, Jasleen, Serrano, Juan C, Bailer, Peter F, Parker, Jared B, Evitt, Niklaus H, Gajula, Kiran S, Shi, Junwei, Kohli, Rahul M

“…Abstract The partnership of DNA deaminase enzymes with CRISPR-Cas nucleases is now a well-established method to enable targeted genomic base editing. However,…”
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Olefin Isomerization Regiochemistries during Tandem Action of BacA and BacB on Prephenate in Bacilysin Biosynthesis by Parker, Jared B, Walsh, Christopher T

“…BacA and BacB, the first two enzymes of the bacilysin pathway, convert prephenate to an exocylic regioisomer of dihydrohydroxyphenylpyruvate (ex-H2HPP) on the…”
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Stereochemical Outcome at Four Stereogenic Centers during Conversion of Prephenate to Tetrahydrotyrosine by BacABGF in the Bacilysin Pathway by Parker, Jared B, Walsh, Christopher T

“…The first four enzymes of the bacilysin antibiotic pathway, BacABGF, convert prephenate to a tetrahydrotyrosine (H4Tyr) diastereomer on the way to the…”
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Uracil DNA Glycosylase: Revisiting Substrate-Assisted Catalysis by DNA Phosphate Anions by Parker, Jared B, Stivers, James T

“…Uracil DNA glycosylase (UNG) is a powerful DNA repair enzyme that has been shown to stabilize a glycosyl cation reaction intermediate and a related tight…”
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Expression and purification of cell-penetrating Cas9 and Cas12a enzymes for peptide-assisted genome editing by Cuomo, Rosella G, Zhang, Zhen, Yamada, Keisuke, Krosky, Alexander J, Shi, Junwei, Kohli, Rahul M, Parker, Jared B

“…Recent advances in CRISPR-Cas genomic editors have shifted us ever closer to achieving the ultimate therapeutic goal of accomplishing any edit in any cell…”
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Uracil DNA Glycosylase: Revisiting Substrate-Assisted Catalysis by DNA Phosphate Anions super([dagger]) by Stivers, James T, Parker, Jared B

“…Uracil DNA glycosylase (UNG) is a powerful DNA repair enzyme that has been shown to stabilize a glycosyl cation reaction intermediate and a related tight…”
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Enzymatic Capture of a Transient Extrahelical Thymine in the Search for Uracil in DNA by Parker, Jared B., Bianchet, Mario A., Krosky, Daniel J., Friedman, Joshua I., Amzel, L. Mario, Stivers, James T.

“…The enzyme uracil DNA glycosylase (UNG) excises unwanted uracil bases in the genome using an extrahelical base recognition mechanism. Efficient removal of…”
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