Susceptibility of wild and colonized Anopheles stephensi to Plasmodium vivax infection

As much as 80% of global Plasmodium vivax infections occur in South Asia and there is a shortage of direct studies on infectivity of P. vivax in Anopheles stephensi, the most common urban mosquito carrying human malaria. In this quest, the possible effects of laboratory colonization of mosquitoes on...

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Bibliographic Details
Published in:	Malaria journal Vol. 17; no. 1; p. 225
Main Authors:	Mohanty, Ajeet Kumar, Nina, Praveen Balabaskaran, Ballav, Shuvankar, Vernekar, Smita, Parkar, Sushma, D'souza, Maria, Zuo, Wenyun, Gomes, Edwin, Chery, Laura, Tuljapurkar, Shripad, Valecha, Neena, Rathod, Pradipsinh K, Kumar, Ashwani
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 05-06-2018 BioMed Central BMC
Subjects:	Animals Anopheles Anopheles - parasitology Anopheles albimanus Anopheles gambiae Anopheles stephensi Aquatic insects Bacteria Colonization Colonized populations Culicidae Development and progression Disease susceptibility Drinking water Female Females Genes Genetic diversity Health aspects Human diseases Humidity India Infections Infectivity Laboratories Larvae Malaria Microbiota Mosquito Vectors - parasitology Mosquitoes Oocysts Parasites Plasmodium Plasmodium falciparum Plasmodium vivax Plasmodium vivax - isolation & purification Population Pupae Sporozoites Vector-borne diseases Wild populations India South Asia
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Summary:	As much as 80% of global Plasmodium vivax infections occur in South Asia and there is a shortage of direct studies on infectivity of P. vivax in Anopheles stephensi, the most common urban mosquito carrying human malaria. In this quest, the possible effects of laboratory colonization of mosquitoes on infectivity and development of P. vivax is of interest given that colonized mosquitoes can be genetically less divergent than the field population from which they originated. Patient-derived P. vivax infected blood was fed to age-matched wild and colonized An. stephensi. Such a comparison requires coordinated availability of same-age wild and colonized mosquito populations. Here, P. vivax infection are studied in colonized An. stephensi in their 66th-86th generation and fresh field-caught An. stephensi. Wild mosquitoes were caught as larvae and pupae and allowed to develop into adult mosquitoes in the insectary. Parasite development to oocyst and sporozoite stages were assessed on days 7/8 and 12/13, respectively. While there were batch to batch variations in infectivity of individual patient-derived P. vivax samples, both wild and colonized An. stephensi were roughly equally susceptible to oocyst stage Plasmodium infection. At the level of sporozoite development, significantly more mosquitoes with sporozoite load of 4+ were seen in wild than in colonized populations.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1475-2875 1475-2875
DOI:	10.1186/s12936-018-2343-0