Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus

Plants are promising host systems for recombinant protein production. However, progress in the commercialization of plant-made proteins (PMPs) has been slow. Only one PMP drug is commercially available. In this study, we explored the possibility of using plants to produce E2 of classical swine fever...

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Published in:	Journal of plant biology = Singmul Hakhoe chi Vol. 61; no. 4; pp. 241 - 252
Main Authors:	Sohn, Eun-Ju, Lee, Yongjik, Park, Namjo, Park, Minhee, Kim, Nam Hyung, Park, Soohong, Min, Kyungmin, Gu, Sungmin, Park, Youngmin, Song, Jaeyoung, An, Dong-jun, Hwang, Inhwan
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2018 Springer Nature B.V 한국식물학회
Subjects:	Antibodies Antigens Antisera Biomedical and Life Sciences Cellulose Cellulosic resins Commercialization E2 protein Expression vectors Fever Fusion protein Hog cholera Host plants Host systems Immunization Life Sciences Original Article Plant Breeding/Biotechnology Plant Ecology Plant Genetics and Genomics Plant Sciences Plant Systematics/Taxonomy/Biogeography Protein purification Proteins Purification Swine Transgenic plants Vaccines Viruses 생물학 Green vaccine Molecular farming Classical swine fever virus (CSFV) Plant-made proteins (PMPs)
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Summary:	Plants are promising host systems for recombinant protein production. However, progress in the commercialization of plant-made proteins (PMPs) has been slow. Only one PMP drug is commercially available. In this study, we explored the possibility of using plants to produce E2 of classical swine fever virus (CSFV) and the use of this plant-produced E2 as a vaccine. We designed high-level expression vectors for transgenic plants by considering the transcription, translation, and storage of E2 in the cell. We incorporated a cellulosebinding domain sequence into the expression vector as an affinity tag for cost-effective, one-step purification. Using this vector, we generated multiple lines of transgenic Arabidopsis thaliana plants expressing a fusion protein of E2 from CSFV at high levels (0.7% of total soluble proteins). ER-targeted E2 fusion protein was successfully purified via a one-step purification process using amorphous cellulose resin. Arabidopsis-produced E2 was recognized by an antibody that detects CSFV antigen. Finally, antisera from mice immunized with E2 fusion protein reacted strongly to the antigens in a CSFV antibody detection kit. Therefore, we propose that plant-produced E2 fusion proteins could be further developed for use as a green vaccine against CSFV in animals.
ISSN:	1226-9239 1867-0725
DOI:	10.1007/s12374-018-0133-4