Search Results - "Pardo, J.P."
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Development of new h CaM-Alexa Fluor ® biosensors for a wide range of ligands
Published in Analytical biochemistry (01-01-2017)Get full text
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Development of new hCaM-Alexa Fluor® biosensors for a wide range of ligands
Published in Analytical biochemistry (01-01-2017)“…Eight new fluorescent biosensors of human calmodulin (hCaM) using Alexa Fluor® 350, 488, 532, and 555 dyes were constructed. These biosensors are…”
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The mitochondrial respiratory chain of Ustilago maydis
Published in Biochimica et biophysica acta (04-10-2004)“…Ustilago maydis mitochondria contain the four classical components of the electron transport chain (complexes I, II, III, and IV), a glycerol phosphate…”
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Functional properties of the Ustilago maydis alternative oxidase under oxidative stress conditions
Published in Mitochondrion (01-04-2009)“…The mitochondrial respiratory chain of Ustilago maydis contains two terminal oxidases, the cytochrome c oxidase (COX) and the alternative oxidase (AOX). To…”
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Effect of calcium and calmodulin on RNA synthesis in isolated nuclei from rat liver cells
Published in FEBS letters (21-06-1982)“…The authors investigated the effects of calcium, calmodulin and the calmodulin inhibitor, chlorpromazine, on RNA synthesis in isolated nuclei from rat liver…”
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Chronic intake of moderate fat-enriched diet induces fatty liver and low-grade inflammation without obesity in rabbits
Published in Chemico-biological interactions (25-02-2019)“…Non-Alcoholic Fatty Liver Disease (NAFLD) is the cause of chronic liver disease. Even though NAFLD is strongly associated with obesity and metabolic syndrome,…”
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Rapid Purification and Biochemical Characterization of Glucose Kinase from Streptomyces peucetius var. caesius
Published in Archives of biochemistry and biophysics (15-10-2001)“…Glucose kinase catalyzes the ATP-dependent phosphorylation of glucose. Streptomyces peucetius var. caesius glucose kinase was purified 292-fold to homogeneity…”
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Trehalose-mediated protection of the plasma membrane H+-ATPase from Kluyveromyces lactis during freeze-drying and rehydration
Published in Cryobiology (01-09-1998)“…During freeze-drying and rehydration, the activity of the H+-ATPase from the plasma membrane of Kluyveromyces lactis was preserved by increasing concentrations…”
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A Novel ATP-diphosphohydrolase from Human Term Placental Mitochondria
Published in Placenta (Eastbourne) (01-07-1999)“…This report describes an ATP-diphosphohydrolase activity associated with the inner membrane of human term placental mitochondria. An enriched fraction…”
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Reactive cysteines of the yeast plasma-membrane H+-ATPase (PMA1): mapping the sites of inactivation by N-ethylmaleimide
Published in The Journal of biological chemistry (17-01-1997)“…We have taken advantage of cysteine mutants described previously (Petrov, V. V., and Slayman, C. W. (1995) J. Biol. Chem. 270, 28535-28540) to map the sites at…”
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Reactivity of the H +-ATPase from Kluyveromyces lactis to Sulfhydryl Reagents
Published in Archives of biochemistry and biophysics (01-08-1995)“…N-Ethylmaleimide (NEM) inhibited the H +-ATPase (EC 3.6.1.35) from Kluyveromyces lactis with a second-rate constant of 200 M −1 min −1. H +-ATPase was…”
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Reactivity of the H+-ATPase form Kluyveromyces lactis to sulfhydryl reagents
Published in Archives of biochemistry and biophysics (01-08-1995)“…N-Ethylmaleimide (NEM) inhibited the H+-ATPase (EC 3.6.1.35) from Kluyveromyces lactis with a second rate constant of 200 M-1 min-1. H+-ATPase was partially…”
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Interactions of calcium with yeast mitochondria
Published in Cell calcium (Edinburgh) (01-04-1992)“…The interactions of Ca2+ with mitochondria from Saccharomyces cerevisiae were explored. Mitochondria were loaded with the metallochromic dye Fluo-3 to measure…”
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Characterization of the F 1F 0-ATPase and the tightly-bound ATPase activities in submitochondrial particles from human term placenta
Published in Placenta (Eastbourne) (1996)“…In a previous study we demonstrated the existence of a tightly-bound ATPase in the human placental mitochondria (Martínez et al., 1993). The current study…”
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Denaturing Behavior of Glutathione Reductase from Cyanobacterium Spirulina maxima in Guanidine Hydrochloride
Published in Archives of biochemistry and biophysics (20-04-1995)“…The influence of guanidine hydrochloride (Gdn-HCl) on glutathione reductase from Spirulina maxima has been studied by measuring the changes in enzymatic…”
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Characterization of the F1F0-ATPase and the tightly-bound ATPase activities in submitochondrial particles from human term placenta
Published in Placenta (Eastbourne) (01-07-1996)“…In a previous study we demonstrated the existence of a tightly-bound ATPase in the human placental mitochondria (Martínez et al., 1993). The current study…”
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The fluorescein isothiocyanate-binding site of the plasma-membrane H+-ATPase of Neurospora crassa
Published in The Journal of biological chemistry (15-12-1988)“…The mammalian (Na+,K+), Ca2+-, and (H+,K+)-ATPases contain a well-characterized lysine residue that reacts with fluorescein 5′-isothiocyanate (FITC); enzymatic…”
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The use of a cyanine dye in measuring membrane potential in yeast
Published in Archives of biochemistry and biophysics (15-05-1984)“…An attempt was made to use 3,3'-dipropylthiacarbocyanine as a membrane potential probe in yeast by following both its fluorescence changes and its uptake by…”
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Cysteine 532 and Cysteine 545 Are the N-ethylmaleimide-reactive Residues of the Neurospora Plasma Membrane H+-ATPase
Published in The Journal of biological chemistry (05-06-1989)“…Previous studies from this laboratory (Brooker, R. J., and Slayman, C. W. (1983) J. Biol. Chem. 258, 222–226; Davenport, J. W., and Slayman, C. W. (1988) J…”
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Early metabolic effects and mechanism of ammonium transport in yeast
Published in Archives of biochemistry and biophysics (01-03-1987)“…Studies were performed to define the effects and mechanism of NH+4 transport in yeast. The following results were obtained. Glucose was a better facilitator…”
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