Cytotoxicity and antibacterial activity of scutellarein and carajurone-enriched fraction obtained from the hydroethanolic extract of the leaves of Fridericia chica (Bonpl.) L.G. Lohmann

Cytotoxicity and antibacterial activity of scutellarein and carajurone-enriched fraction obtained from the hydroethanolic extract of the leaves of Fridericia chica (Bonpl.) L.G. Lohmann

The hydroethanolic extract obtained from the dry leaves of Fridericia chica (HEFc) underwent several fractionations by different chromatographic techniques. The ethyl acetate and dichloromethane fraction were subjected to phytochemical analysis, resulting in the identification and isolation of scute...

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Bibliographic Details
Published in:Natural product research Vol. 35; no. 23; pp. 5287 - 5293
Main Authors: Violante, Ivana M. P., Carollo, Carlos A., Silva, Larissa I., Oliveira, Adna Q. C., Pardinho, Flavia C., Garcez, Walmir Silva, Garcez, Fernanda R., Oliveira, Ruberlei Godinho de, Arunachalam, Karuppusamy, de Oliveira Martins, Domingos Tabajara
Format: Journal Article
Language:English
Published: England Taylor & Francis 02-12-2021
Taylor & Francis Ltd
Subjects:
Acetic acid
antibacterial
Antibacterial activity
carajurone
Chromatography
Cytotoxicity
Dichloromethane
Ethyl acetate
Fridericia chica
Membrane permeability
Mode of action
Nucleotides
Pseudomonas aeruginosa
scutellarein
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus pyogenes
Toxicity testing
Fridericia chica
antibacterial
carajurone
scutellarein
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Summary:The hydroethanolic extract obtained from the dry leaves of Fridericia chica (HEFc) underwent several fractionations by different chromatographic techniques. The ethyl acetate and dichloromethane fraction were subjected to phytochemical analysis, resulting in the identification and isolation of scutellarein (1) and in a fraction rich in carajurone (2). They were tested for cytotoxicity in CHO-K1 and the antibacterial activity and mode of action by in vitro assays. The HEFc and scutellarein (1) presented no cytotoxicity. The results showed good antibacterial effect of HEFc against Streptococcus pyogenes and Staphylococcus aureus and moderate activity for Staphylococcus epidermidis, Pseudomonas aeruginosa and Salmonella typhimurium. The fraction containing the compound carajurone (2) showed good activity against Staphylococcus aureus and Staphylococcus epidermidis and moderate activity against Streptococcus pyogenes. Scutellarein (1) showed no activity against the bacteria tested. HEFc antibacterial mode of action appeared to be associated with changes in the permeability of bacterial membranes and nucleotide leakage.
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ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2020.1753050