Photophysical and biological assessment of coumarin-6 loaded polymeric nanoparticles as a cancer imaging agent

Polymeric nanoparticles can prove beneficial in oncology as they sidestep limitations associated with traditional small-molecule pharmaceuticals. With respect to drug delivery, polymeric nanoparticles possess structural features that make them suitable as carriers for therapy and imaging contrast, a...

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Bibliographic Details
Published in:	Sensors & diagnostics Vol. 2; no. 5; pp. 1277 - 1285
Main Authors:	Gregoriou, Yiota, Gregoriou, Gregoria, Manoli, Andreas, Papageorgis, Paris, Mc Larney, Benedict, Vangeli, Despoina, McColman, Sarah, Yilmaz, Vural, Hsu, Hsiao-ting, Skubal, Magdalena, Ogirala, Anuja, Athanasiou, Evangelia, Cramb, David T, Dietis, Nikolas, Strati, Katerina, Itskos, Grigorios, Constantinou, Andreas I, Andreou, Chrysafis
Format:	Journal Article
Language:	English
Published:	14-09-2023
Online Access:	Get full text
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Summary:	Polymeric nanoparticles can prove beneficial in oncology as they sidestep limitations associated with traditional small-molecule pharmaceuticals. With respect to drug delivery, polymeric nanoparticles possess structural features that make them suitable as carriers for therapy and imaging contrast, as their core/shell morphology offers an effective method of transport that provides increased tumor access with reduced side effects, especially for drugs with low water solubility. Herein, we present a polymeric nanoparticle made from pluronic F127 and vitamin E-TPGS encapsulating coumarin-6 for the optical imaging of cancer. We explored the biophysical properties of the construct using an array of optical and physical techniques, evaluated its uptake in breast cancer cell lines, the in vivo toxicokinetics in zebrafish, and its biodistribution profile in mouse xenograft models bearing PC3 tumors overexpressing the prostate-specific membrane antigen (PSMA). The biophysical characterization of the nanoformulation, combined with its selective uptake by cancer cells, its low in vivo toxicity profile, and effective tumor targeting demonstrate the versatility and potential of this nanoparticle formulation for drug delivery applications. Coumarin-6 is used as a model hydrophobic drug to investigate delivery to tumors via polymeric micelles. Photophysical characterization, as well as in vitro and in vivo experiments showcase this platform for cancer diagnosis and imaging.
Bibliography:	https://doi.org/10.1039/d3sd00065f Electronic supplementary information (ESI) available. See DOI
ISSN:	2635-0998 2635-0998
DOI:	10.1039/d3sd00065f