The efficacy of retrograde infusion with LY231617 in a rat middle cerebral artery occlusion model

We examined the efficacy of the antioxidant LY231617 administered five hours following middle cerebral artery (MCA) occlusion in rats. The treatment was contrived for a two hour interval. Group A (n = 16) was left untreated. Group B (n = 16) received an intravenous infusion of LY231617. Group C (n =...

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Bibliographic Details
Published in:	Canadian journal of neurological sciences Vol. 23; no. 3; p. 175
Main Authors:	Inoue, N, Yamamoto, Y L, Ito, Y, Clemens, J A, Panetta, J K, Diksic, M
Format:	Journal Article
Language:	English
Published:	England 01-08-1996
Subjects:	Animals Antioxidants - pharmacology Autoradiography Brain Ischemia - drug therapy Butylated Hydroxytoluene - analogs & derivatives Butylated Hydroxytoluene - pharmacology Cerebral Arteries - drug effects Cerebrovascular Circulation - drug effects Disease Models, Animal Rats Rats, Sprague-Dawley
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Summary:	We examined the efficacy of the antioxidant LY231617 administered five hours following middle cerebral artery (MCA) occlusion in rats. The treatment was contrived for a two hour interval. Group A (n = 16) was left untreated. Group B (n = 16) received an intravenous infusion of LY231617. Group C (n = 16) received saline (86 microliters/min) by retrograde infusion of the cerebral vein (RICV). Group D (n = 22) was administered LY231617 (10mg/kg/2 hr) in saline (86 microliters/min) by RICV. Local cerebral blood flow with [14C]-iodoantipyrine and blood-brain transfer constant with 14C-alpha-amino-isobutyric acid were examined. Early ischemic damage was histologically examined with cresyl violet and Luxol fast blue and with triphenyl-tetrazolium chloride. The results revealed a marked increase in local cerebral blood flow (over 600%, p < 0.01) after RICV with LY231617, with a significant improvement of BBB permeability in rats from group D. Ischemic brain damage measured with Luxol fast blue and triphenyl-tetrazolium chloride methods showed a significant improvement (50-91%) of ischemic damage in group D, as compared to groups B and C. Retrograde infusion of the cerebral vein with LY231617 resulted in a significant amelioration at seven hours post MCA occlusion.
ISSN:	0317-1671
DOI:	10.1017/S0317167100038476