Carbon fiber based electrochemical sensor for sweat cortisol measurement

Carbon fiber based electrochemical sensor for sweat cortisol measurement

This study examines the use of a conductive carbon fiber to construct a flexible biosensing platform for monitoring biomarkers in sweat. Cortisol was chosen as a model analyte. Functionalization of the conductive carbon yarn (CCY) with ellipsoidal Fe 2 O 3 has been performed to immobilize the antibo...

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Bibliographic Details
Published in:Scientific reports Vol. 9; no. 1; p. 403
Main Authors: Sekar, M., Pandiaraj, M., Bhansali, S., Ponpandian, N., Viswanathan, C.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 23-01-2019
Nature Publishing Group
Subjects:
140/133
639/925/357/551
692/700/139/1735
Antibodies
Biosensors
Carbodiimide
Carbon
Carbon fibers
Chemiluminescence
Cortisol
Electrochemistry
Fourier analysis
Fourier transforms
Hormones
Humanities and Social Sciences
Infrared spectroscopy
Mechanical properties
multidisciplinary
Raman spectroscopy
Scanning electron microscopy
Science
Science (multidisciplinary)
Sensors
Sweat
X-ray diffraction
X-ray spectroscopy
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Summary:This study examines the use of a conductive carbon fiber to construct a flexible biosensing platform for monitoring biomarkers in sweat. Cortisol was chosen as a model analyte. Functionalization of the conductive carbon yarn (CCY) with ellipsoidal Fe 2 O 3 has been performed to immobilize the antibodies specific to cortisol. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) chemistry has been used to immobilize the antibodies onto the Fe 2 O 3 modified CCY. Crystallinity, structure, morphology, flexibility, surface area, and elemental analysis were studied using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, Field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FE-SEM/EDS) and Brunauer–Emmett–Teller (BET) analysis. Mechanical properties of the fiber such as tensile strength, young’s modulus have also been investigated. Under optimal parameters, the fabric sensor exhibited a good linearity (r 2  = 0.998) for wide a linear range from 1 fg to 1 μg with a detection limit of 0.005 fg/mL for the sensitive detection of cortisol. Repeatability, reliability, reproducibility, and anti-interference properties of the current sensor have been investigated. Detection of cortisol levels in human sweat samples has also been investigated and the results were validated with commercial chemiluminescence immunoassay (CLIA) method.
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content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-37243-w