Evaluation of Granulysin and Perforin as Candidate Biomarkers for Protection Following Vaccination with Mycobacterium bovis BCG or M. bovisΔRD1

Evaluation of Granulysin and Perforin as Candidate Biomarkers for Protection Following Vaccination with Mycobacterium bovis BCG or M. bovisΔRD1

The development of improved vaccines against tuberculosis (TB) is directly linked to the investigation of new and better correlates of protection after vaccination against TB. Cloning and characterization of bovine homologues of the antimicrobial protein granulysin (Bo-lysin) and perforin by our gro...

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Bibliographic Details
Published in:Transboundary and emerging diseases Vol. 56; no. 6-7; pp. 228 - 239
Main Authors: Capinos Scherer, Charles F, Endsley, Janice J, de Aguiar, Juliana B, Jacobs Jr, William R, Larsen, Michelle H, Palmer, Mitchell.V, Nonnecke, Brian J, Ray Waters, W, Mark Estes, D
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-08-2009
Blackwell Publishing Ltd
Subjects:
biomarker
granulysin
IFNγ
perforin
tuberculosis
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Summary:The development of improved vaccines against tuberculosis (TB) is directly linked to the investigation of new and better correlates of protection after vaccination against TB. Cloning and characterization of bovine homologues of the antimicrobial protein granulysin (Bo-lysin) and perforin by our group could be used as potential biomarkers for TB vaccination efficacy. In the present study, we examined the kinetics of granulysin, perforin, IFNγ and Fas-L responses to Mycobacterium bovis purified protein derivative (PPD) stimulation by peripheral blood mononuclear cells from M. bovisΔRD1-, BCG- and non-vaccinated cattle. Gene expression profiles following PPD stimulation showed significant increases in transcripts for granulysin and IFNγ in both CD4⁺ and CD8⁺ T cells in BCG-vaccinated as compared with non-vaccinated animals. Perforin and IFNγ examined by flow cytometry, showed a difference of 1-2% more PPD-specific cells in BCG-vaccinated than non-vaccinated animals. In the vaccine trial, granulysin and perforin were significantly increased in both vaccine groups as compared with control after vaccination and challenge. IFNγ expression was increased only after vaccination and secretion was higher in the control, non-protected group as compared with both vaccine groups demonstrating no correlation with protection upon vaccination. In summary, results shown here provide evidence that granulysin and perforin are prospective candidates as biomarkers of protection after vaccination against TB.
Bibliography:http://dx.doi.org/10.1111/j.1865-1682.2008.01058.x
istex:2B30A4FDDF8ED4346F166C81F3660224DAE074A5
ArticleID:TBED1058
ark:/67375/WNG-SPJHPC2F-R
ISSN:1865-1674
1865-1682
DOI:10.1111/j.1865-1682.2008.01058.x