Gastroprotective action of the ethanol extract of Leonurus sibiricus L. (Lamiaceae) in mice

Leonurus sibiricus L. (Lamiaceae) is a medicinal plant known in Brazil as “rubim” or “erva de macaé”. It is used for various purposes, including stomach disorders. To evaluate the effect of the ethanol extract of the aerial parts of L. sibiricus (EELs) in models of gastric damage in mice. The effect...

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Bibliographic Details
Published in:	Journal of ethnopharmacology Vol. 284; p. 114792
Main Authors:	Biano, Laiza S., Oliveira, Alan S., Palmeira, David N., Silva, Luis André, de Albuquerque-Junior, Ricardo L.C., Duarte, Marcelo C., Correa, Cristiane B., Grespan, Renata, Batista, Josemar S., Camargo, Enilton A.
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier B.V 10-02-2022
Subjects:	Animals Enzyme Inhibitors - pharmacology Ethanol - toxicity Ethylmaleimide - pharmacology Gastric mucosa Gastric ulcer Gene Expression Regulation - drug effects Glyburide - pharmacology Hypoglycemic Agents - pharmacology Leonurus - chemistry Leonurus sibiricus L Male Medicinal plants Mice Nitric Oxide - metabolism Oxidative stress Phytotherapy Plant Extracts - chemistry Plant Extracts - pharmacology Potassium Channels - genetics Potassium Channels - metabolism Prostaglandins - genetics Prostaglandins - metabolism Random Allocation Stomach Ulcer - prevention & control Sulfhydryl Compounds - metabolism Medicinal plants Oxidative stress NO L-NAME Gastric mucosa EELS Glibenc H2S Gastric ulcer T80/W NP-SH KATP NEM PAG Leonurus sibiricus L SEM
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Summary:	Leonurus sibiricus L. (Lamiaceae) is a medicinal plant known in Brazil as “rubim” or “erva de macaé”. It is used for various purposes, including stomach disorders. To evaluate the effect of the ethanol extract of the aerial parts of L. sibiricus (EELs) in models of gastric damage in mice. The effect of EELs (50, 100 and 300 mg/kg, p.o., 1 h before induction) was tested on acidified ethanol (ACEt)-induced gastric ulcers. Additionally, we tested the effect of EELs (by intraduodenal administration) in the pylorus ligation (PL) model. Pretreatment with EELs, at 300 mg/kg, but not 50 and 100 mg/kg, reduced the relative area of gastric ulcers induced by ACEt (p < 0.01) and lipoperoxidation (p < 0.001), and increased the sulfhydryl content (p < 0.01) in the stomach in comparison with the vehicle group. Pretreatment with N-ethylmaleimide (a blocker of non-protein sulfhydryl groups, 10 mg/kg, i.p.) or glibenclamide (a KATP channel blocker, 10 mg/kg, i.p.) inhibited the gastroprotective response caused by EELs (300 mg/kg; p < 0.001), but there were no alterations due to pretreatments with inhibitors of the synthesis of prostaglandins (indomethacin, 10 mg/kg), nitric oxide (L-NAME, 70 mg/kg) or hydrogen sulfide (DL-propargylglycine, 10 mg/kg). Treatment with EELs (300 mg/kg) reduced mucus production (p < 0.001) and the volume of gastric secretion (p < 0.001) after PL without affecting gastric acidity or pH. These results provide evidence that EELs exerts gastroprotective action in mice, with the participation of oxidative stress and mediation of NP-SH, KATP channels and mucus production. [Display omitted]
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-8741 1872-7573
DOI:	10.1016/j.jep.2021.114792