Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V

Mlc1p is a calmodulin‐like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP‐like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth an...

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Bibliographic Details
Published in:	Acta crystallographica. Section D, Biological crystallography. Vol. 58; no. 10-2; pp. 1882 - 1885
Main Authors:	Terrak, Mohammed, Otterbein, Ludovic R., Wu, Guanming, Palecanda, Lakshmi A., Lu, Renne C., Dominguez, Roberto
Format:	Journal Article Web Resource
Language:	English
Published:	5 Abbey Square, Chester, Cheshire CH1 2HU, England Munksgaard International Publishers 01-10-2002 Blackwell Publishing
Subjects:	Amino Acid Sequence Binding Sites Biochemistry, biophysics & molecular biology Biochimie, biophysique & biologie moléculaire calmodulin-like proteins Cell Division Cloning, Molecular Escherichia coli Life sciences Mlc1p Models, Molecular Molecular Sequence Data Myosin Light Chains - chemistry Myosin Light Chains - isolation & purification Myosin Light Chains - metabolism Myosin Light Chains/chemistry/isolation & purification/metabolism Myosin Type V - chemistry Myosin Type V - metabolism Myosin Type V/chemistry/metabolism Protein Conformation purification/metabolism Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Recombinant Proteins/chemistry/isolation & purification/metabolism Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - isolation & purification Saccharomyces cerevisiae Proteins - metabolism Saccharomyces cerevisiae Proteins/chemistry/isolation Saccharomyces cerevisiae/growth & development/physiology Sciences du vivant Support, U.S. Gov't, Non-P.H.S Support, U.S. Gov't, P.H.S X-Ray Diffraction - methods
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Summary:	Mlc1p is a calmodulin‐like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP‐like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2–IQ3 (referred to as IQ2,3). Electron‐density maps for two of the complexes (Mlc1p–IQ4 and Mlc1p–IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p–IQ2 and Mlc1p–IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p–IQ2,3 as a search model.
Bibliography:	ark:/67375/WNG-D7X9DG71-3 istex:DCCAA570C9B2B1F20FDBF346C1220C50F6A99FDF ArticleID:AYDPU0063 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 scopus-id:2-s2.0-0036793039
ISSN:	1399-0047 0907-4449 1399-0047
DOI:	10.1107/S0907444902013951