Search Results - "Palczewska, Małgorzata"

An efficient method for the construction of artificial, concatemeric DNA, RNA and proteins with genetically programmed functions, using a novel, vector-enzymatic DNA fragment amplification-expression technology by Skowron, Piotr M., Krawczun, Natalia, Żebrowska, Joanna, Krefft, Daria, Żołnierkiewicz, Olga, Bielawa, Marta, Jeżewska-Frąckowiak, Joanna, Janus, Łukasz, Witkowska, Małgorzata, Palczewska, Małgorzata, Zylicz-Stachula, Agnieszka

“…De novo designed bioactive molecules, such as DNA, RNA and peptides, are utilized in increasingly diverse scientific, industrial and biomedical applications…”
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Temperature dependence of ligand-protein complex formation as reflected by saturation transfer difference NMR experiments by Groves, Patrick, Kövér, Katalin E., André, Sabine, Bandorowicz-Pikula, Joanna, Batta, Gyula, Bruix, Marta, Buchet, René, Canales, Angeles, Cañada, F. Javier, Gabius, Hans-Joachim, Laurents, Douglas V., Naranjo, Jose R., Palczewska, Małgorzata, Pikula, Slawomir, Rial, Eduardo, Strzelecka-Kiliszek, Agnieszka, Jiménez-Barbero, Jesús

“…We show that temperature is an important parameter for the sensitivity of saturation transfer difference (STD) spectroscopy. A decreased intensity of STD…”
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Stimulators of mineralization limit the invasive phenotype of human osteosarcoma cells by a mechanism involving impaired invadopodia formation by Cmoch, Anna, Podszywalow-Bartnicka, Paulina, Palczewska, Malgorzata, Piwocka, Katarzyna, Groves, Patrick, Pikula, Slawomir

“…Osteosarcoma (OS) is a highly aggressive bone cancer affecting children and young adults. Growing evidence connects the invasive potential of OS cells with…”
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Use of Pichia pastoris for the Expression, Purification, and Characterization of Rat Calretinin “EF-Hand” Domains by Palczewska, Małgorzata, Groves, Patrick, Kuźnicki, Jacek

“…Calretinin (CR) is a calcium-binding, neuronal protein of undefined function. Related proteins either buffer intracellular calcium concentrations or are…”
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Data regarding a new, vector-enzymatic DNA fragment amplification-expression technology for the construction of artificial, concatemeric DNA, RNA and proteins, as well as biological effects of selected polypeptides obtained using this method by Skowron, Piotr M., Krawczun, Natalia, Żebrowska, Joanna, Krefft, Daria, Żołnierkiewicz, Olga, Bielawa, Marta, Jeżewska-Frąckowiak, Joanna, Janus, Łukasz, Witkowska, Małgorzata, Palczewska, Małgorzata, Schumacher, Adriana, Wardowska, Anna, Deptuła, Milena, Czupryn, Artur, Mucha, Piotr, Piotrowski, Arkadiusz, Sachadyn, Paweł, Rodziewicz-Motowidło, Sylwia, Pikuła, Michał, Zylicz-Stachula, Agnieszka

“…Applications of bioactive peptides and polypeptides are emerging in areas such as drug development and drug delivery systems. These compounds are bioactive,…”
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Neutralization of cholera toxin by Rosaceae family plant extracts by Komiazyk, Magdalena, Palczewska, Malgorzata, Sitkiewicz, Izabela, Pikula, Slawomir, Groves, Patrick

“…Cholera is one of the most deadly diarrheal diseases that require new treatments. We investigated the neutralization of cholera toxin by five plant extracts…”
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Diffusion nuclear magnetic resonance spectroscopy detects substoichiometric concentrations of small molecules in protein samples by Ribeiro, João P., Palczewska, Małgorzata, André, Sabine, Cañada, F. Javier, Gabius, Hans-Joachim, Jiménez-Barbero, Jesús, Mellström, Britt, Naranjo, José R., Scheffers, Dirk-Jan, Groves, Patrick

“…Small molecules are difficult to detect in protein solutions, whether they originate from elution during affinity chromatography (e.g., imidazole, lactose),…”
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Bacterial type AB₅ enterotoxins--structure, function and mechanism of action by Komiażyk, Magdalena, Palczewska, Małgorzata, Pikula, Sławomir, Groves, Patrick

“…One of the main factors causing bacterial diarrhea are AB₅ enterotoxins. This group is divided into four families: pertussis toxin, cholera toxin, shiga toxin…”
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Characterization of calretinin I–II as an EF‐hand, Ca2+, H+‐sensing domain by Palczewska, Małgorzata, Batta, Gyula, Groves, Patrick, Linse, Sara, Kuźnicki, Jacek

“…Calretinin, a neuronal protein with well‐defined calcium‐binding properties, has a poorly defined function. The pH dependent properties of calretinin (CR), the…”
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Use of plant extracts to control and treat AB5 enterotoxin-related diarrhea by Komiazyk, Magdalena, Palczewska, Małgorzata, Sitkiewicz, Izabela, Groves, Patrick

“…Plants contain a broad spectrum of small molecules with potential antimicrobial properties. Here, we review the antimicrobial activities of plant extracts…”
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S100A proteins in propagation of a calcium signal in norm and pathology by Cmoch, Anna, Groves, Patrick, Palczewska, Małgorzata, Pikuła, Sławomir

“…Calcium ions are essential factors controlling the balance between cell survival, growth, differentiation and metabolism. Ca2+ acts as a global second…”
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Characterization of calretinin I–II as an EF‐hand, Ca 2+ , H + ‐sensing domain by Palczewska, Małgorzata, Batta, Gyula, Groves, Patrick, Linse, Sara, Kuźnicki, Jacek

“…Abstract Calretinin, a neuronal protein with well‐defined calcium‐binding properties, has a poorly defined function. The pH dependent properties of calretinin…”
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Calretinin and calbindin D28k have different domain organizations by Palczewska, Małgorzata, Groves, Patrick, Batta, Gyula, Heise, Bert, Kuźnicki, Jacek

“…The domain organization of calretinin (CR) was predicted to involve all six EF‐hand motifs (labeled I to VI) condensed into a single domain, as characterized…”
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Transcriptional repressor DREAM regulates trigeminal noxious perception by Benedet, Tomaso, Gonzalez, Paz, Oliveros, Juan C., Dopazo, Jose M., Ghimire, Kedar, Palczewska, Malgorzata, Mellstrom, Britt, Naranjo, Jose R.

“…Expression of the downstream regulatory element antagonist modulator (DREAM) protein in dorsal root ganglia and spinal cord is related to endogenous control…”
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Protein molecular weight standards can compensate systematic errors in diffusion-ordered spectroscopy by Groves, Patrick, Palczewska, Małgorzata, Dolores Molero, M, Batta, Gyula, Javier Cañada, F, Jiménez-Barbero, Jesús

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Matrix vesicles isolated from mineralization-competent Saos-2 cells are selectively enriched with annexins and S100 proteins by Cmoch, Anna, Strzelecka-Kiliszek, Agnieszka, Palczewska, Malgorzata, Groves, Patrick, Pikula, Slawomir

“…► Functional MVs of Saos-2 cells are enriched in annexins A1, A2, A6 and A7. ► Some annexin levels in MVs change upon stimulation for mineralization. ► Annexin…”
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Cation binding properties of calretinin, an EF-hand calcium-binding protein by Groves, P, Palczewska, M

“…Calretinin (CR) is a neuronal EF-hand protein previously characterized as a calcium (micromolar affinity) binding protein. CR-containing neurons are spared in…”
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Sumoylation regulates nuclear localization of repressor DREAM by Palczewska, Malgorzata, Casafont, Iñigo, Ghimire, Kedar, Rojas, Ana M., Valencia, Alfonso, Lafarga, Miguel, Mellström, Britt, Naranjo, Jose R.

“…DREAM is a Ca2+-binding protein with specific functions in different cell compartments. In the nucleus, DREAM acts as a transcriptional repressor, although the…”
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Concanavalin A-agarose removes mannan impurities from an extracellularly expressed Pichia pastoris recombinant protein by Palczewska, Małgorzata, Batta, Gyula, Groves, Patrick

“…Pichia pastoris secretes few native proteins. However, the more than 1 g l(-1)of extracellularly expressed mannan interfered with the purification of our…”
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Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression by Savignac, Magali, Pintado, Belen, Gutierrez-Adan, Alfonso, Palczewska, Malgorzata, Mellström, Britt, Naranjo, Jose R

“…Downstream Regulatory Element Antagonist Modulator (DREAM) is a Ca2+‐dependent transcriptional repressor expressed in the brain, thyroid gland and thymus…”
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