Three tRNA Binding Sites in Rabbit Liver Ribosomes and Role of the Intrinsic ATPase in 80S Ribosomes from Higher Eukaryotes

Three tRNA binding sites have been found in organisms of all domains (former kingdoms) with only one exception: Four binding sites have been reported for cytoplasmic 80S ribosomes from rabbit liver. Therefore, the issue was reconsidered, and the data revealed that rabbit liver ribosomes contain thr...

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Bibliographic Details
Published in:	Biochemistry (Easton) Vol. 36; no. 34; pp. 10492 - 10497
Main Authors:	El'skaya, Anna V, Ovcharenko, Galina V, Palchevskii, Sergey S, Petrushenko, Zoja M, Triana-Alonso, Francisco J, Nierhaus, Knud H
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 26-08-1997
Subjects:	Adenosine Triphosphatases - metabolism Adenosine Triphosphate - metabolism Animals Binding Sites Kinetics Liver - chemistry Liver - metabolism Magnesium - pharmacology Nucleotides - pharmacology Peptide Biosynthesis Peptides - metabolism Rabbits Ribosomal Proteins - metabolism Ribosomes - chemistry Ribosomes - enzymology Ribosomes - metabolism RNA, Messenger - metabolism RNA, Transfer - metabolism RNA, Transfer, Phe - metabolism Spermine - pharmacology
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Summary:	Three tRNA binding sites have been found in organisms of all domains (former kingdoms) with only one exception: Four binding sites have been reported for cytoplasmic 80S ribosomes from rabbit liver. Therefore, the issue was reconsidered, and the data revealed that rabbit liver ribosomes contain three tRNA binding sites, underlining the universal character of this ribosomal feature. Furthermore, a first analysis of the role of the ribosome intrinsic ATPase was performed. This ATPase is found in ribosomes of higher eukarya but not in lower eukarya such as yeast or ribosomes of the domains archea and bacteria. The results suggest that the intrinsic ATPase fulfills the same function as the essential third elongation factor EF-3, an ATPase in higher fungi (yeast etc.), that facilitates the release of the deacylated tRNA from the E site.
Bibliography:	Abstract published in Advance ACS Abstracts, August 15, 1997. istex:4488E4FBF627AC6E7FB45185E17EEBA319B4F3F4 A.V.E. was supported by ISF Grant UBA 200 and SCST of Ukraine Grant 5.3/16. ark:/67375/TPS-BP7LXKLQ-L ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0006-2960 1520-4995
DOI:	10.1021/bi970631e