Search Results - "Palacky, Jan"
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Systematic investigation of sequence requirements for DNA i-motif formation
Published in Nucleic acids research (18-03-2019)“…Abstract The formation of intercalated motifs (iMs) - secondary DNA structures based on hemiprotonated C.C+ pairs in suitable cytosine-rich DNA sequences, is…”
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CD Study of the G-Quadruplex Conformation
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2019)“…Circular Dichroic (CD) spectroscopy is one of the most frequently used methods for guanine quadruplex studies and in general for studies of conformational…”
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Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study
Published in Nucleic acids research (01-01-2013)“…DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K(+)-stabilized human telomere quadruplexes by…”
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Does Raman spectroscopy recognize different G‐quadruplex arrangements?
Published in Journal of Raman spectroscopy (01-02-2020)“…In this work, we demonstrate the underused potential of Raman spectroscopy in detecting formation of DNA G‐quadruplex (G4) and differentiating between various…”
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5
DNA i-motif formation at neutral pH is driven by kinetic partitioning
Published in Nucleic acids research (11-04-2023)“…Abstract Cytosine-rich DNA regions can form four-stranded structures based on hemi-protonated C.C+ pairs, called i-motifs (iMs). Using CD, UV absorption, NMR…”
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DNA i‐motif formation at physiological pH revealed by Raman spectroscopy
Published in Journal of Raman spectroscopy (01-01-2024)“…The intercalated motif (iM) is a four‐stranded structure consisting of two parallel homoduplexes formed by hemiprotonated C.C+ pairs and intercalated…”
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G‐Quadruplex Formation by DNA Sequences Deficient in Guanines: Two Tetrad Parallel Quadruplexes Do Not Fold Intramolecularly
Published in Chemistry : a European journal (19-08-2021)“…Guanine quadruplexes (G4s) are noncanonical forms of nucleic acids that are frequently found in genomes. The stability of G4s depends, among other factors, on…”
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Influence of Silicate Rock Glass Compositions on the Efficacy of Prebiotic RNA Polymerization Reactions: The Case of 3’,5’ Cyclic Guanosine Monophosphate
Published in ChemSystemsChem (01-09-2023)“…Silicate glasses are ubiquitous on terrestrial planets wherever molten rock – generated by volcanism or impacts – is quenched by air or water. Hence, they…”
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Pressure effects reveal that changes in the redox states of the heme iron complexes in the sensor domains of two heme‐based oxygen sensor proteins, EcDOS and YddV, have profound effects on their flexibility
Published in The FEBS journal (01-12-2014)“…The catalytic activity of a heme‐based oxygen sensor phosphodiesterase from Escherichia coli (EcDOS) towards cyclic diGMP is regulated by the redox state of…”
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Raman Microspectroscopy of the Yeast Vacuoles
Published in Journal of spectroscopy (Hindawi) (01-01-2012)“…In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was…”
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SVD-based method for intensity normalization, background correction and solvent subtraction in Raman spectroscopy exploiting the properties of water stretching vibrations
Published in Journal of Raman spectroscopy (01-07-2011)“…A semiautomated method combining intensity normalization with effective elimination of the solvent signal and non‐Raman background is presented for Raman…”
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Analysis of composite nanofibrous layers by confocal Raman microscopy
Published in Polymer (Guilford) (26-09-2014)“…A method for the determination of nanofibrous mats chemical composition based on Raman spectroscopy and singular value decomposition is presented. Two…”
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13
Pressure effects reveal that changes in the redox states of the heme iron complexes in the sensor domains of two heme‐based oxygen sensor proteins, Ec DOS and YddV, have profound effects on their flexibility
Published in The FEBS journal (01-12-2014)“…The catalytic activity of a heme‐based oxygen sensor phosphodiesterase from Escherichia coli ( Ec DOS ) towards cyclic di GMP is regulated by the redox state…”
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