Search Results - "Palacián, E."

Pitfalls in the use of carboxylic acid anhydrides for structural studies of nucleoprotein particles by Nieto, M A, Palacián, E

“…During modification of protein amino groups with carboxylic acid anhydrides, these reagents cause a fall in pH, which can be prevented by addition of base…”
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Interaction with RNA polymerase of nucleosomal cores lacking one H2A.H2B dimer by GONZALEZ, P. J, MARTINEZ, C, PALACIAN, E

“…Nucleosomal particles lacking one H2A.H2B dimer interact with RNA polymerase from Escherichia coli more strongly than the complete nucleosomal core particles…”
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Transcription of mononucleosomal particles acetylated in the presence of n-butyrate by PIÑEIRO, M, HERNANDEZ, F, PUERTA, C, PALACIÁN, E

“…Although a correlation between chemical acetylation of the amino-terminal tails of core histones and stimulation of RNA synthesis has been reported for…”
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Dimethylmaleic anhydride, a specific reagent for protein amino groups by de la Escalera, S, Palacián, E

“…The reagent dimethylmaleic anhydride does not cause a stable modification of thiol compounds under the conditions used for modification of protein amino…”
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Transcription of DNA templates associated with histone (H3 x H4)(2) tetramers by Chirinos, M, Hernández, F, Palacián, E

“…To investigate the in vitro transcription by bacteriophage T7 RNA polymerase of oligonucleosomes lacking histone H2A x H2B dimers, templates were assembled…”
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Solubilization of membrane-bound transpeptidase from Streptomyces strain K-11 with 2,3-dimethylmaleic anhydride by Palacián, E

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Repressive Effect on Oligonucleosome Transcription of the Core Histone Tail Domains by Chirinos, Mayel, Hernández, Francisco, Palacián, Enrique

“…Histone−DNA templates for bacteriophage T7 RNA polymerase were assembled from histone octamers and three different DNA species, two circular (pGEMEX-1 and…”
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Effects of different amino-group reagents on ribosomal integrity: structural role of lysine residues by VIOQUE, A, HERNANDEZ, F, PALACIAN, E

“…Treatment of 60S subunits from yeast ribosomes with dicarboxylic acid anhydrides (maleic, dimethylmaleic and tetrahydrophtalic), which introduces…”
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Properties and function of yeast pyruvate carboxylase by Ruiz-Amil, M, De Torrontegui, G, Palacián, E, Catalina, L, Losada, M

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Modification of the lysine residues of histones H1 and H5: effects on structure and on the binding to chromatin by JORDANO, J, BARBERO, J. L, MONTERO, F, PALACIAN, E

“…The extensive modification of histone H1 from calf thymus with the amino-group reagent dimethylmaleic anhydride (over 35 lysine residues modified per molecule)…”
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Protein-deficient ribosomal particles from yeast 60S subunits obtained by modification with dimethylmaleic anhydride and by treatment with NH4Cl by Vioque, A, Palacián, E

“…The protein-deficient particles prepared from yeast 60S subunits by modification of lysine residues with the reversible reagent dimethylmaleic anhydride are…”
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Interconversion of the active and inactive forms of Chlorella nitrate reductase by Moreno, C.G., Aparicio, P.J., Palacián, E., Losada, M.

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Inhibition of yeast pyruvate carboxylase by L-aspartate and oxaloacetate by Palacián, E, de Torrontegui, G, Losada, M

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Acetylation of Histone H2A.H2B Dimers Facilitates Transcription by Puerta, C., Hernandez, F., Lopezalarcon, L., Palacian, E.

“…Histone-DNA templates for bacteriophage T7 RNA polymerase were assembled from a plasmid containing a promoter and a terminator for T7 RNA polymerase, intact…”
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Interaction of RNA polymerase II with structurally altered nucleosomal particles: transcription is facilitated by loss of one H2A•H2B dimer by GONZALEZ, P. J, PALACIAN, E

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Modification of 50S ribosomal subunits with N-bromosuccinimide by López-Rivas, A, Pintor-Toro, J A, Hernández, F, Palacián, E

“…The 50S subunits of Escherichia coli ribosomes were modified with the tryptophan reagent N-bromosuccinimide, and the sulfhydryl groups, the modification of…”
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Interaction of RNA polymerase II with structurally altered nucleosomal particles. Transcription is facilitated by loss of one H2A.H2B dimer by P J González, E Palacián

“…The loss of one H2A.H2B dimer from the nucleosomal core increases its affinity for RNA polymerase II and its efficiency as a transcription template, allowing…”
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Studies on the kinetic mechanism of nitrate reductase from spinach (Spinacea oleracea) by de la Rosa, F F, Palacián, E, Castillo, F

“…Based on Lineweaver-Burk plots of the initial velocities, at different concentrations of NADH and nitrate, and product inhibition patterns, an Iso Ping Pong Bi…”
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Transcriptional Properties of Oligonucleosomal Templates Containing Acetylated (H3·H4) 2 Tetramers by Hernandez, F., Puerta, C., Lopezalarcon, L., Palacian, E.

“…Direct chemical acetylation of an oligonucleosomal template for bacteriophage T7 RNA polymerase is accompanied by a substantial increase in its capability to…”
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Yeast nucleosomal particles: structural and transcriptional properties by Pineiro, Manuel, Puerta, Carmen, Palacian, Enrique

“…Yeast nucleosomal core particles have been characterized by thermal denaturation, circular dichroism, and digestion with DNase I and with trypsin. Practically…”
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