Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria

We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance gene mecA in Staphylococcus epide...

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Bibliographic Details
Published in:	Lab on a chip Vol. 12; no. 2; p. 333
Main Authors:	Liedert, Ralph, Amundsen, Lotta K, Hokkanen, Ari, Mäki, Minna, Aittakorpi, Anne, Pakanen, Mikko, Scherer, James R, Mathies, Richard A, Kurkinen, Marika, Uusitalo, Sanna, Hakalahti, Leena, Nevanen, Tarja K, Siitari, Harri, Söderlund, Hans
Format:	Journal Article
Language:	English
Published:	England 21-01-2012
Subjects:	Bacterial Proteins - genetics Bacterial Proteins - metabolism DNA, Bacterial - analysis Drug Resistance, Bacterial - genetics Electrophoresis, Agar Gel Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microscopy, Confocal Polymethyl Methacrylate - chemistry Staphylococcus epidermidis - genetics
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Summary:	We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance gene mecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.
ISSN:	1473-0189
DOI:	10.1039/c1lc20782b