Validation of analytical method for (E)-2-decenedioic acid quantification in honey samples

[Display omitted] •Establishment of marker compound ((E)-2-decenedioic acid) that can distinguish natural honey and sugar-fed honey.•Establishment of an analytical method for (E)-2-decenedioic acid in honeys by UPLC.•Validation of the UPLC method by linearity, limit of detection, limit of quantifica...

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Bibliographic Details
Published in:	Journal of Asia-Pacific entomology Vol. 24; no. 4; pp. 1153 - 1157
Main Authors:	Kim, Se Gun, Kim, Hyo Young, Kim, Seonmi, Choi, Hong Min, Pak, Sok Chun, Han, Sang Mi
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-12-2021 한국응용곤충학회
Subjects:	Composition Natural honey Sugar-fed honey Ultra-performance liquid chromatography Validation 농학 Validation Sugar-fed honey Composition Natural honey Ultra-performance liquid chromatography
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Summary:	[Display omitted] •Establishment of marker compound ((E)-2-decenedioic acid) that can distinguish natural honey and sugar-fed honey.•Establishment of an analytical method for (E)-2-decenedioic acid in honeys by UPLC.•Validation of the UPLC method by linearity, limit of detection, limit of quantification, precision and accuracy tests.•More amount in sugar-fed honey compared to natural honey contend (E)-2-decenedioic acid. Natural honey is a highly sought-after product owing to its biological uniqueness. We developed a suitable analytical method for the quantitative analysis of (E)-2-decenedioic acid in Korean natural and sugar-fed honey samples. A total of 258 honey samples were screened using ultra-performance liquid chromatography (UPLC) for the detection and quantification of (E)-2-decenedioic acid. (E)-2-decenedioic acid was present in high concentrations (122–127 mg/kg) in sugar-fed honey but only in trace amounts (10.9–14.8 mg/kg) in natural honey. Findings indicate that the UPLC method is suitable and reliable for the quantitative evaluation of (E)-2-decenedioic acid in honey. We expect that this method will be applicable for detecting honey adulteration.
ISSN:	1226-8615 1876-7990
DOI:	10.1016/j.aspen.2021.10.013