Search Results - "Pak, Ryan A."

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response by Adamson, Britt, Norman, Thomas M., Jost, Marco, Cho, Min Y., Nuñez, James K., Chen, Yuwen, Villalta, Jacqueline E., Gilbert, Luke A., Horlbeck, Max A., Hein, Marco Y., Pak, Ryan A., Gray, Andrew N., Gross, Carol A., Dixit, Atray, Parnas, Oren, Regev, Aviv, Weissman, Jonathan S.

“…Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq,…”
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Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation by Horlbeck, Max A, Gilbert, Luke A, Villalta, Jacqueline E, Adamson, Britt, Pak, Ryan A, Chen, Yuwen, Fields, Alexander P, Park, Chong Yon, Corn, Jacob E, Kampmann, Martin, Weissman, Jonathan S

“…We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive…”
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Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis by Lee, Sebum, Shang, Yulei, Redmond, Stephanie A., Urisman, Anatoly, Tang, Amy A., Li, Kathy H., Burlingame, Alma L., Pak, Ryan A., Jovičić, Ana, Gitler, Aaron D., Wang, Jinhua, Gray, Nathanael S., Seeley, William W., Siddique, Teepu, Bigio, Eileen H., Lee, Virginia M.-Y., Trojanowski, John Q., Chan, Jonah R., Huang, Eric J.

“…Persistent accumulation of misfolded proteins causes endoplasmic reticulum (ER) stress, a prominent feature in many neurodegenerative diseases including…”
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Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors by Replogle, Joseph M, Bonnar, Jessica L, Pogson, Angela N, Liem, Christina R, Maier, Nolan K, Ding, Yufang, Russell, Baylee J, Wang, Xingren, Leng, Kun, Guna, Alina, Norman, Thomas M, Pak, Ryan A, Ramos, Daniel M, Ward, Michael E, Gilbert, Luke A, Kampmann, Martin, Weissman, Jonathan S, Jost, Marco

“…CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial…”
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Chemical mapping of the surface interactome of PIEZO1 identifies CADM1 as a modulator of channel inactivation by Koster, Anna K, Yarishkin, Oleg, Dubin, Adrienne E, Kefauver, Jennifer M, Pak, Ryan A, Cravatt, Benjamin F, Patapoutian, Ardem

“…The propeller-shaped blades of the PIEZO1 and PIEZO2 ion channels partition into the plasma membrane and respond to indentation or stretching of the lipid…”
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Adaptive exchange sustains cullin-RING ubiquitin ligase networks and proper licensing of DNA replication by Zhang, Yaru, Jost, Marco, Pak, Ryan A, Lu, Daniel, Li, Jing, Lomenick, Brett, Garbis, Spiros D, Li, Chi-Ming, Weissman, Jonathan S, Lipford, James Russell, Deshaies, Raymond J

“…Cop9 signalosome (CSN) regulates the function of cullin-RING E3 ubiquitin ligases (CRLs) by deconjugating the ubiquitin-like protein NEDD8 from the cullin…”
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