Anthelmintic activity of nanoencapsulated carvacryl acetate against gastrointestinal nematodes of sheep and its toxicity in rodents

The objective of this study was to evaluate the efficacy of carvacryl acetate (CVA) and nanoencapsulated CVA (nCVA) on gastrointestinal nematodes of sheep. The CVA was nanoencapsulated with chitosan/gum arabic and the efficacy of nanoencapsulation (EE), yield, zeta potential, nanoparticle morphology...

Full description

Saved in:

Bibliographic Details
Published in:	Revista brasileira de parasitologia veterinaria Vol. 29; no. 1; p. e013119
Main Authors:	André, Weibson Paz Pinheiro, Paiva Junior, José Ribamar de, Cavalcante, Géssica Soares, Ribeiro, Wesley Lyeverton Correia, Araújo Filho, José Vilemar de, Santos, Jéssica Maria Leite Dos, Alves, Ana Paula Negreiros Nunes, Monteiro, Jomar Patrício, Morais, Selene Maia de, Silva, Isaac Neto Goes da, Oliveira, Lorena Mayana Beserra de, Abreu, Flávia Oliveira Monteiro da Silva, Bevilaqua, Claudia Maria Leal
Format:	Journal Article
Language:	English
Published:	Brazil Colégio Brasileiro de Parasitologia Veterinária 2020 Colégio Brasileiro de Parasitologia Veterinaria
Subjects:	acetylation Animals Anthelmintics - administration & dosage Anthelmintics - toxicity biocompounds Chitosan Feces - parasitology Female Gastrointestinal Tract - parasitology gum arabic Haemonchus contortus Mice Monoterpenes - administration & dosage Monoterpenes - toxicity Nanocapsules - administration & dosage Nanocapsules - toxicity Nematoda - classification Nematoda - drug effects Nematoda - isolation & purification Nematode Infections - drug therapy Nematode Infections - parasitology Parasite Egg Count Parasitic Sensitivity Tests PARASITOLOGY Real-Time Polymerase Chain Reaction Rodentia Sheep Sheep Diseases - drug therapy Sheep Diseases - parasitology Toxicity Tests toxicological safety VETERINARY SCIENCES acetylation goma arábica Haemonchus contortus Quitosana acetilação biocompostos toxicological safety gum arabic biocompounds Chitosan segurança toxicológica
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The objective of this study was to evaluate the efficacy of carvacryl acetate (CVA) and nanoencapsulated CVA (nCVA) on gastrointestinal nematodes of sheep. The CVA was nanoencapsulated with chitosan/gum arabic and the efficacy of nanoencapsulation (EE), yield, zeta potential, nanoparticle morphology and release kinetics at pH 3 and 8 were analyzed. Acute and subchronic toxicity were evaluated in rodents and reduction of egg counts in the faeces (FECRT) of sheep. The sheep were divided into four groups (n = 10): G1, 250 mg/kg CVA; G2, 250 mg/kg nCVA; G3, polymer matrix and G4: 2.5 mg/kg monepantel. EE and nCVA yield were 65% and 57%, respectively. The morphology of the nanoparticles was spherical, size (810.6±286.7 nm), zeta potential in pH 3.2 (+18.3 mV) and the 50% release of CVA at pHs 3 and 8 occurred at 200 and 10 h, respectively. nCVA showed LD50 of 2,609 mg/kg. CVA, nCVA and monepantel reduced the number of eggs per gram of faeces (epg) by 57.7%, 51.1% and 97.7%, respectively. The epg of sheep treated with CVA and nCVA did not differ from the negative control (P>0.05). Nanoencapsulation reduced the toxicity of CVA; however, nCVA and CVA presented similar results in the FECRT.
ISSN:	0103-846X 1984-2961 1984-2961
DOI:	10.1590/s1984-29612019098