Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes

Significant amounts of cell wall degrading (CWD) enzymes are required to degrade lignocellulosic biomass into its component sugars. One strategy for reducing exogenous enzyme production requirements is to produce the CWD enzymes in planta . For this work, various CWD enzymes were expressed in maize...

Full description

Saved in:

Bibliographic Details
Published in:	Bioenergy research Vol. 4; no. 4; pp. 276 - 286
Main Authors:	Zhang, Dongcheng, VanFossen, Amy L., Pagano, Ryan M., Johnson, Jeremy S., Parker, Matthew H., Pan, Shihao, Gray, Benjamin N., Hancock, Elaina, Hagen, Daniel J., Lucero, Héctor A., Shen, Binzhang, Lessard, Philip A., Ely, Cairn, Moriarty, Meghan, Ekborg, Nathan A., Bougri, Oleg, Samoylov, Vladimir, Lazar, Gabor, Raab, R. Michael
Format:	Journal Article
Language:	English
Published:	New York Springer-Verlag 01-12-2011 Springer Springer Nature B.V
Subjects:	Alternative energy sources Analysis Biodiesel fuels Biofuels Biomass Biomass energy Biomedical and Life Sciences Biotechnology Cellulose Corn Costs Dextrose Enzymes Ethanol Ethyl alcohol Fermentation Food Genetic engineering Genetically engineered foods Genetically modified crops Glucose Hydrolysis Life Sciences Lignocellulose Maize Plant biomass Plant Breeding/Biotechnology Plant Ecology Plant Genetics and Genomics Plant Sciences Plants (organisms) Raw materials Saccharomyces cerevisiae Stover Studies Sugar Transgenic Transgenic plants Wood Science & Technology Yeasts Zea mays United States > US Hydrolysis Yeast Corn stover Enzyme Simultaneous saccharification and fermentation (SSF) Biomass Pretreatment Biofuels Fermentation Cell wall Genetic modification
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Significant amounts of cell wall degrading (CWD) enzymes are required to degrade lignocellulosic biomass into its component sugars. One strategy for reducing exogenous enzyme production requirements is to produce the CWD enzymes in planta . For this work, various CWD enzymes were expressed in maize ( Zea mays ). Following growth and dry down of the plants, harvested maize stover was tested to determine the impact of the expressed enzymes on the production of glucose and xylose using different exogenous enzyme loadings. In this study, a consolidated pretreatment and hydrolysis process consisting of a moderate chemical pretreatment at temperatures below 75°C followed by enzymatic hydrolysis using an in-house enzyme cocktail was used to evaluate engineered transgenic feedstocks. The carbohydrate compositional analysis showed no significant difference in the amounts of glucan and xylan between the transgenic maize plants expressing CWD enzyme(s) and the control plants. Hydrolysis results demonstrated that transgenic plants expressing CWD enzymes achieved up to 141% higher glucose yield and 172% higher xylose yield over the control plants from enzymatic hydrolysis under the experimental conditions. The hydrolytic performance of a specific xylanase (XynA) expressing transgenic event (XynA.2015.05) was heritable in the next generation, and the improved properties can be achieved even with a 25% reduction in exogenous enzyme loading. Simultaneous saccharification and fermentation of biomass hydrolysates from two different transgenic maize lines with yeast ( Saccharomyces cerevisiae D5A) converted 65% of the biomass glucan into ethanol, versus only a 42% ethanol yield with hydrolysates from control plants, corresponding to a 55% improvement in ethanol production.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1939-1234 1939-1242
DOI:	10.1007/s12155-011-9138-2