Comparison of 1% cyclosporine eye drops in olive oil and in linseed oil to treat experimentally-induced keratoconjunctivitis sicca in rabbits

To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two vehicles-olive and linseed oil-and that of the oils themselves in treating experimentally-induced keratoconjunctivitis sicca (KCS) in rabbits. KCS was induced in 25 New Zealand rabbits using 1% atropine s...

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Published in:	Arquivos brasileiros de oftalmologia Vol. 78; no. 5; pp. 295 - 299
Main Authors:	Parrilha, Leticia Rodrigues, Nai, Gisele Alborghetti, Giuffrida, Rogério, Barbero, Rafael Cabral, Padovani, Leticia Dias Fabris, Pereira, Ricardo Henrique Zaquir, Silva, Danielle Alves, Silva, Mariele Catherine Alves, Diniz, Miriely Stein, Andrade, Silvia Franco
Format:	Journal Article
Language:	English
Published:	Brazil Conselho Brasileiro de Oftalmologia 01-09-2015
Subjects:	Administration, Ophthalmic Animals Conjunctiva - drug effects Conjunctiva - pathology Cornea - drug effects Cornea - pathology Cyclosporine - administration & dosage Cyclosporine - chemistry Drug Carriers - administration & dosage Drug Combinations Fluorescein Goblet Cells - drug effects Immunosuppressive Agents - administration & dosage Immunosuppressive Agents - chemistry Keratoconjunctivitis Sicca - drug therapy Keratoconjunctivitis Sicca - pathology Linseed Oil - administration & dosage Male Olive Oil - administration & dosage Ophthalmic Solutions - administration & dosage Ophthalmic Solutions - chemistry OPHTHALMOLOGY Rabbits Reproducibility of Results Tears - secretion Treatment Outcome Rabbits Coelhos Ciclosporina/administração & dosagem Linseed oil Keratoconjunctivitis sicca Ceratoconjuntivite seca Cyclosporine/administration & dosage Óleo de semente do linho Olive oil Óleo de palmeira
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Summary:	To evaluate the effectiveness of topical 1% cyclosporine eye drops diluted in either of the two vehicles-olive and linseed oil-and that of the oils themselves in treating experimentally-induced keratoconjunctivitis sicca (KCS) in rabbits. KCS was induced in 25 New Zealand rabbits using 1% atropine sulfate eye drops for 7 days before treatment and throughout the treatment period (12 weeks). The rabbits were divided into five groups: one control (C) group without KCS induction and four treatment groups in which KCS was induced and treated topically with olive oil (O), linseed oil (L), cyclosporine in olive oil (CO), and cyclosporine in linseed oil (CL). The animals were evaluated using Schirmer tear test 1 (STT), the fluorescein test (FT), tear-film break-up time (TBUT), the rose bengal test (RBT), and histopathological analysis. Values of STT and TBUT significantly decreased 1 week post-induction (p<0.05) and were similar to initial values after the 4th week of treatment, in all groups. After KCS induction, there was significantly less corneal damage in group L than in group CL, as assessed FT and RBT. Histopathology demonstrated that Groups L and CL presented less edema and corneal congestion. There was no significant difference in the goblet cell density (cells/mm2) between the groups (p=0.147). Cyclosporine diluted in olive oil or linseed oil was effective in the treatment of KCS, although it had better efficacy when diluted in linseed oil. Linseed oil presented better effectiveness, whether associated or not, than olive oil. These results may contribute to the creation of novel topical ophthalmic formulations for KCS treatment in future.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0004-2749 1678-2925 1678-2925
DOI:	10.5935/0004-2749.20150078