Search Results - "Pacheco, Sabino"

CRISPR-Cas9 knockout of membrane-bound alkaline phosphatase or cadherin does not confer resistance to Cry toxins in Aedes aegypti by Pacheco, Sabino, Gallegos, Adrián S, Peláez-Aguilar, Ángel E, Sánchez, Jorge, Gómez, Isabel, Soberón, Mario, Bravo, Alejandra

“…The Aedes aegypti cadherin-like protein (Aae-Cad) and the membrane-bound alkaline phosphatase (Aae-mALP) are membrane proteins identified as putative receptors…”
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Whole Genome Sequencing Analysis of Bacillus thuringiensis GR007 Reveals Multiple Pesticidal Protein Genes by Pacheco, Sabino, Gómez, Isabel, Chiñas, Marcos, Sánchez, Jorge, Soberón, Mario, Bravo, Alejandra

“…Bacillus thuringiensis ( Bt ) are soil ubiquitous bacteria. They produce a great variability of insecticidal proteins, where certain of these toxins are used…”
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In vivo nanoscale analysis of the dynamic synergistic interaction of Bacillus thuringiensis Cry11Aa and Cyt1Aa toxins in Aedes aegypti by López-Molina, Samira, do Nascimento, Nathaly Alexandre, Silva-Filha, Maria Helena Neves Lobo, Guerrero, Adán, Sánchez, Jorge, Pacheco, Sabino, Gill, Sarjeet S, Soberón, Mario, Bravo, Alejandra

“…The insecticidal Cry11Aa and Cyt1Aa proteins are produced by Bacillus thuringiensis as crystal inclusions. They work synergistically inducing high toxicity…”
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Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins) by Correa, Agustín, Pacheco, Sabino, Mechaly, Ariel E, Obal, Gonzalo, Béhar, Ghislaine, Mouratou, Barbara, Oppezzo, Pablo, Alzari, Pedro M, Pecorari, Frédéric

“…Glycosidases are associated with various human diseases. The development of efficient and specific inhibitors may provide powerful tools to modulate their…”
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Rearrangement of N-Terminal α-Helices of Bacillus thuringiensis Cry1Ab Toxin Essential for Oligomer Assembly and Toxicity by Pacheco, Sabino, Quiliche, Jean Piere Jesus, Gómez, Isabel, Sánchez, Jorge, Soberón, Mario, Bravo, Alejandra

“…Cry proteins produced by are pore-forming toxins that disrupt the membrane integrity of insect midgut cells. The structure of such pore is unknown, but it has…”
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The expression of Longus type 4 pilus of enterotoxigenic Escherichia coli is regulated by LngR and LngS and by H‐NS, CpxR and CRP global regulators by De la Cruz, Miguel A., Ruiz‐Tagle, Alejandro, Ares, Miguel A., Pacheco, Sabino, Yáñez, Jorge A., Cedillo, Lilia, Torres, Javier, Girón, Jorge A.

“…Summary Enterotoxigenic Escherichia coli produces a long type 4 pilus called Longus. The regulatory elements and the environmental signals controlling the…”
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Additional regulatory activities of MrkH for the transcriptional expression of the Klebsiella pneumoniae mrk genes: Antagonist of H-NS and repressor by Ares, Miguel A, Fernández-Vázquez, José L, Pacheco, Sabino, Martínez-Santos, Verónica I, Jarillo-Quijada, Ma Dolores, Torres, Javier, Alcántar-Curiel, María D, González-Y-Merchand, Jorge A, De la Cruz, Miguel A

“…Klebsiella pneumoniae is a common opportunistic pathogen causing nosocomial infections. One of the main virulence determinants of K. pneumoniae is the type 3…”
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Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence by Chimal-Cázares, Fernando, Hernández-Martínez, Gabriela, Pacheco, Sabino, Ares, Miguel A, Soria-Bustos, Jorge, Sánchez-Gutiérrez, Manuel, Izquierdo-Vega, Jeannett A, Ibarra, Jose Antonio, González-Y-Merchand, Jorge A, Gorvel, Jean-Pierre, Méresse, Stéphane, De la Cruz, Miguel A

“…serotype Typhimurium is a bacterium that causes gastroenteritis and diarrhea in humans. The genome of Typhimurium codes for diverse virulence factors, among…”
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Transcriptional Profiling of Type II Toxin-Antitoxin Genes of Helicobacter pylori under Different Environmental Conditions: Identification of HP0967-HP0968 System by Cárdenas-Mondragón, María G, Ares, Miguel A, Panunzi, Leonardo G, Pacheco, Sabino, Camorlinga-Ponce, Margarita, Girón, Jorge A, Torres, Javier, De la Cruz, Miguel A

“…is a Gram-negative bacterium that colonizes the human gastric mucosa and is responsible for causing peptic ulcers and gastric carcinoma. The expression of…”
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Dominant negative mutants of Bacillus thuringiensis Cry1Ab toxin function as anti-toxins: demonstration of the role of oligomerization in toxicity by Rodríguez-Almazán, Claudia, Zavala, Luis Enrique, Muñoz-Garay, Carlos, Jiménez-Juárez, Nuria, Pacheco, Sabino, Masson, Luke, Soberón, Mario, Bravo, Alejandra

“…Bacillus thuringiensis Cry toxins, that are used worldwide in insect control, kill insects by a mechanism that depends on their ability to form oligomeric…”
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Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins by Fernández, Luisa Elena, Gómez, Isabel, Pacheco, Sabino, Arenas, Iván, Gilla, Sarjeet S., Bravo, Alejandra, Soberón, Mario

“…Phage display is an in vitro method for selecting polypeptides with desired properties from a large collection of variants. The insecticidal Cry toxins…”
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Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage by Pacheco, Sabino, Gómez, Isabel, Sato, Ryoichi, Bravo, Alejandra, Soberón, Mario

“…The Cry1Ac toxin from Bacillus thuringiensis was displayed on the surface of T7 phage. The cry1Ac gene was fused to the C-terminal end of T7-10B capsid protein…”
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A major conformational change of N‐terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity by Pacheco, Sabino, Gómez, Isabel, Soberón, Mario, Bravo, Alejandra

“…Pore forming toxins rely on oligomerization for membrane insertion to kill their targets. Bacillus thuringiensis produces insecticidal Cry‐proteins composed of…”
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Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis) by Wang, Zeyu, Fang, Longfa, Zhou, Zishan, Pacheco, Sabino, Gómez, Isabel, Song, Fuping, Soberón, Mario, Zhang, Jie, Bravo, Alejandra

“…The bacterium Bacillus thuringiensis produces several insecticidal proteins, such as the crystal proteins (Cry) and the vegetative insecticidal proteins (Vip)…”
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A Single Point Mutation Resulting in Cadherin Mislocalization Underpins Resistance against Bacillus thuringiensis Toxin in Cotton Bollworm by Xiao, Yutao, Dai, Qing, Hu, Ruqin, Pacheco, Sabino, Yang, Yongbo, Liang, Gemei, Soberón, Mario, Bravo, Alejandra, Liu, Kaiyu, Wu, Kongming

“…Transgenic plants that produce Bacillus thuringiensis (Bt) crystalline (Cry) toxins are cultivated worldwide to control insect pests. Resistance to B…”
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The Cyt1Aa toxin from Bacillus thuringiensis inserts into target membranes via different mechanisms in insects, red blood cells, and lipid liposomes by Onofre, Janette, Pacheco, Sabino, Torres-Quintero, Mary Carmen, Gill, Sarjeet S., Soberon, Mario, Bravo, Alejandra

“…Bacillus thuringiensis subsp. israelensis produces crystal inclusions composed of three-domain Cry proteins and cytolytic Cyt toxins, which are toxic to…”
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An Intramolecular Salt Bridge in Bacillus thuringiensis Cry4Ba Toxin Is Involved in the Stability of Helix a-3, Which Is Needed for Oligomerization and Insecticidal Activity by Pacheco, Sabino, Gómez, Isabel, Sánchez, Jorge, García-Gómez, Blanca-Ines, Soberón, Mario, Bravo, Alejandra

“…Bacillus thuringiensis three-domain Cry toxins kill insects by forming pores in the apical membrane of larval midgut cells. Oligomerization of the toxin is an…”
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Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity by Torres-Quintero, Mary-Carmen, Gómez, Isabel, Pacheco, Sabino, Sánchez, Jorge, Flores, Humberto, Osuna, Joel, Mendoza, Gretel, Soberón, Mario, Bravo, Alejandra

“…The Cyt and Cry toxins are different pore-forming proteins produced by Bacillus thuringiensis bacteria, and used in insect-pests control. Cry-toxins have a…”
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Functional Bacillus thuringiensis Cyt1Aa Is Necessary To Synergize Lysinibacillus sphaericus Binary Toxin (Bin) against Bin-Resistant and -Refractory Mosquito Species by Nascimento, Nathaly Alexandre, Torres-Quintero, Mary Carmen, Molina, Samira López, Pacheco, Sabino, Romão, Tatiany Patrícia, Pereira-Neves, Antonio, Soberón, Mario, Bravo, Alejandra, Silva-Filha, Maria Helena Neves Lobo

“…The binary (Bin) toxin from is effective to mosquito larvae, but its utilization is threatened by the development of insect resistance. Bin toxin is composed…”
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Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability by Béhar, Ghislaine, Pacheco, Sabino, Maillasson, Mike, Mouratou, Barbara, Pecorari, Frédéric

“…•Affitins are extremophilic affinity proteins derived from the Sac7d family.•We evaluated a rational approach to stabilize a Sac7d derived anti-IgG Affitin.•We…”
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