Search Results - "Pace, N C"

Polar Group Burial Contributes More to Protein Stability than Nonpolar Group Burial by Pace, C. Nick

“…On the basis of studies of Asn to Ala mutants, the gain in stability from burying amide groups that are hydrogen bonded to peptide groups is 80 cal/(mol Å3)…”
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How to measure and predict the molar absorption coefficient of a protein by Pace, C. Nick, Vajdos, Felix, Fee, Lanette, Grimsley, Gerald, Gray, Theronica

“…The molar absorption coefficient, ε, of a protein is usually based on concentrations measured by dry weight, nitrogen, or amino acid analysis. The studies…”
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Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding by Myers, Jeffrey K., Nick Pace, C., Martin Scholtz, J.

“…Denaturant m values, the dependence of the free energy of unfolding on denaturant concentration, have been collected for a large set of proteins. The m value…”
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Charge–charge interactions influence the denatured state ensemble and contribute to protein stability by PACE, C. NICK, ALSTON, ROY W., SHAW, KEVIN L.

“…Several recent studies have shown that it is possible to increase protein stability by improving electrostatic interactions among charged groups on the surface…”
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Conformational stability of globular proteins by Pace, C N

“…The conformational stability of ribonuclease T1 has been measured as a function of the variables of most interest to biochemists: temperature, pH, salt…”
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Measuring and increasing protein stability by Pace, C N

“…Recently, it has become possible to construct proteins to order and this has promoted interest in learning how to increase their stability. This article…”
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Increasing protein stability by altering long-range coulombic interactions by GRIMSLEY, GERALD R., SHAW, KEVIN L., FEE, LANETTE R., ALSTON, ROY W., HUYGHUES-DESPOINTES, BEATRICE M.P., THURLKILL, RICHARD L., SCHOLTZ, J. MARTIN, PACE, C. NICK

“…It is difficult to increase protein stability by adding hydrogen bonds or burying nonpolar surface. The results described here show that reversing the charge…”
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Contribution of the hydrophobic effect to globular protein stability by Pace, C N

“…The decrease in conformational stability, delta(delta G), has been measured for 72 aliphatic side-chain mutants from four proteins in which a larger side-chain…”
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Conformational stability and activity of ribonuclease T1 with zero, one, and two intact disulfide bonds by Pace, C N, Grimsley, G R, Thomson, J A, Barnett, B J

“…Ribonuclease T1 has two disulfide bonds linking cysteine residues 2-10 and 6-103. We have prepared a derivative of ribonuclease T1 in which one disulfide bond…”
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Evaluating contribution of hydrogen bonding and hydrophobic bonding to protein folding by Pace, C N

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Contribution of hydrogen bonding to the conformational stability of ribonuclease T1 by Shirley, Bret A, Stanssens, Patrick, Hahn, Ulrich, Pace, C. Nick

“…For 30 years, the prevailing view has been that the hydrophobic effect contributes considerably more than hydrogen bonding to the conformational stability of…”
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Heat capacity change for ribonuclease A folding by PACE, C. NICK, GRIMSLEY, GERALD R., THOMAS, SUSAN T., MAKHATADZE, GEORGE I.

“…The change in heat capacity ΔCp for the folding of ribonuclease A was determined using differential scanning calorimetry and thermal denaturation curves. The…”
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Protein conformational stabilities can be determined from hydrogen exchange rates by Scholtz, J. Martin, Pace, C. Nick, Huyghues-Despointes, Beatrice M.P

“…Measuring protein conformational stability is one key to solving the protein folding problem. The conformational stability is the free energy change of the…”
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A Helix Propensity Scale Based on Experimental Studies of Peptides and Proteins by Nick Pace, C., Martin Scholtz, J.

“…The average globular protein contains 30% α-helix, the most common type of secondary structure. Some amino acids occur more frequently in α-helices than…”
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Linear extrapolation method of analyzing solvent denaturation curves by Pace, C. Nick, Shaw, Kevin L.

“…The two most common methods of measuring the conformational stability of a protein are differential scanning calorimetry and an analysis of solvent…”
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Cytotoxicity mechanism of the RNase Sa cationic mutants involves inhibition of potassium current through Ca2+-activated channels by Mitkevich, V A, Pace, C N, Koschinski, A, Makarov, A A, Ilinskaya, O N

“…Bacterial ribonucleases (RNases) are considered to be potential anticancer agents. One of most important determinants of RNase cytotoxicity is the net charge…”
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Conformational stability and mechanism of folding of ribonuclease T1 by Thomson, J A, Shirley, B A, Grimsley, G R, Pace, C N

“…Urea and thermal unfolding curves for ribonuclease T1 (RNase T1) were determined by measuring several different physical properties. In all cases, steep,…”
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Urea denaturation of barnase: pH dependence and characterization of the unfolded state by Pace, C. Nick, Laurents, Douglas V, Erickson, Rick E

“…To investigate the pH dependence of the conformational stability of barnase, urea denaturation curves were determined over the pH range 2-10. The maximum…”
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Conformational stability and activity of ribonuclease T1 and mutants. Gln25----Lys, Glu58----Ala, and the double mutant by SHIRLEY, B. A, STANSSENS, P, STEYAERT, J, PACE, C. N

“…Ribonuclease T1 (RNase T1) and mutants Gln25---Lys, Glu58---Ala, and the double mutant were prepared from a chemically synthesized gene, cloned and expressed…”
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Determination and analysis of urea and guanidine hydrochloride denaturation curves by Pace, C N

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