Search Results - "Pace, C. Nick"

Forces stabilizing proteins by Nick Pace, C., Scholtz, J. Martin, Grimsley, Gerald R.

“…The goal of this article is to summarize what has been learned about the major forces stabilizing proteins since the late 1980s when site-directed mutagenesis…”
Get full text

Toward a Molecular Understanding of Protein Solubility: Increased Negative Surface Charge Correlates with Increased Solubility by Kramer, Ryan M., Shende, Varad R., Motl, Nicole, Pace, C. Nick, Scholtz, J. Martin

“…Protein solubility is a problem for many protein chemists, including structural biologists and developers of protein pharmaceuticals. Knowledge about how…”
Get full text

Protein Ionizable Groups: pK Values and Their Contribution to Protein Stability and Solubility by Pace, C. Nick, Grimsley, Gerald R., Scholtz, J. Martin

“…The structure, stability, solubility, and function of proteins depend on their net charge and on the ionization state of the individual residues. Consequently,…”
Get full text

A summary of the measured pK values of the ionizable groups in folded proteins by Grimsley, Gerald R., Scholtz, J. Martin, Pace, C. Nick

“…We tabulated 541 measured pK values reported in the literature for the Asp, Glu, His, Cys, Tyr, and Lys side chains, and the C and N termini of 78 folded…”
Get full text

Contribution of hydrogen bonds to protein stability by Pace, C. Nick, Fu, Hailong, Fryar, Katrina, Landua, John, Trevino, Saul R., Schell, David, Thurlkill, Richard L., Imura, Satoshi, Scholtz, J. Martin, Gajiwala, Ketan, Sevcik, Jozef, Urbanikova, Lubica, Myers, Jeffery K., Takano, Kazufumi, Hebert, Eric J., Shirley, Bret A., Grimsley, Gerald R.

“…Our goal was to gain a better understanding of the contribution of the burial of polar groups and their hydrogen bonds to the conformational stability of…”
Get full text

Contribution of Hydrophobic Interactions to Protein Stability by Pace, C. Nick, Fu, Hailong, Fryar, Katrina Lee, Landua, John, Trevino, Saul R., Shirley, Bret A., Hendricks, Marsha McNutt, Iimura, Satoshi, Gajiwala, Ketan, Scholtz, J. Martin, Grimsley, Gerald R.

“…Our goal was to gain a better understanding of the contribution of hydrophobic interactions to protein stability. We measured the change in conformational…”
Get full text

Protein structure, stability and solubility in water and other solvents by Nick Pace, C., Treviño, Saul, Prabhakaran, Erode, Martin Scholtz, J.

“…Proteins carry out the most difficult tasks in living cells. They do so by interacting specifically with other molecules. This requires that they fold to a…”
Get full text

Amino Acid Contribution to Protein Solubility: Asp, Glu, and Ser Contribute more Favorably than the other Hydrophilic Amino Acids in RNase Sa by Trevino, Saul R., Scholtz, J. Martin, Pace, C. Nick

“…Poor protein solubility is a common problem in high-resolution structural studies, formulation of protein pharmaceuticals, and biochemical characterization of…”
Get full text

pK values of the ionizable groups of proteins by Thurlkill, Richard L., Grimsley, Gerald R., Scholtz, J. Martin, Pace, C. Nick

“…We have used potentiometric titrations to measure the pK values of the ionizable groups of proteins in alanine pentapeptides with appropriately blocked…”
Get full text

Increasing protein stability by improving beta-turns by Fu, Hailong, Grimsley, Gerald R., Razvi, Abbas, Scholtz, J. Martin, Pace, C. Nick

“…Our goal was to gain a better understanding of how protein stability can be increased by improving β‐turns. We studied 22 β‐turns in nine proteins with 66–370…”
Get full text

Urea denatured state ensembles contain extensive secondary structure that is increased in hydrophobic proteins by Nick Pace, C., Huyghues‐Despointes, Beatrice M. P., Fu, Hailong, Takano, Kazufumi, Scholtz, J. Martin, Grimsley, Gerald R.

“…The goal of this article is to gain a better understanding of the denatured state ensemble (DSE) of proteins through an experimental and computational study of…”
Get full text

Energetics of protein hydrogen bonds by Pace, C Nick

“…In this issue, an article gives insight into the microenvironment's influence on the contribution of hydrogen bonds to protein stability…”
Get full text

Peptide Sequence and Conformation Strongly Influence Tryptophan Fluorescence by Alston, Roy W., Lasagna, Mauricio, Grimsley, Gerald R., Scholtz, J. Martin, Reinhart, Gregory D., Pace, C. Nick

“…This article probes the denatured state ensemble of ribonuclease Sa (RNase Sa) using fluorescence. To interpret the results obtained with RNase Sa, it is…”
Get full text

Charge–charge interactions influence the denatured state ensemble and contribute to protein stability by PACE, C. NICK, ALSTON, ROY W., SHAW, KEVIN L.

“…Several recent studies have shown that it is possible to increase protein stability by improving electrostatic interactions among charged groups on the surface…”
Get full text

Tryptophan Fluorescence Reveals the Presence of Long-Range Interactions in the Denatured State of Ribonuclease Sa by Alston, Roy W., Lasagna, Mauricio, Grimsley, Gerald R., Martin Scholtz, J., Reinhart, Gregory D., Nick Pace, C.

“…Characterizing the denatured state ensemble is crucial to understanding protein stability and the mechanism of protein folding. The aim of this research was to…”
Get full text

Charge–charge interactions in the denatured state influence the folding kinetics of ribonuclease Sa by Trefethen, Jared M., Pace, C. Nick, Scholtz, J. Martin, Brems, David N.

“…Gaining a better understanding of the denatured state ensemble of proteins is important for understanding protein stability and the mechanism of protein…”
Get full text

The Contribution of Polar Group Burial to Protein Stability Is Strongly Context-dependent by Takano, Kazufumi, Scholtz, J.Martin, Sacchettini, James C., Pace, C.Nick

“…We previously suggested that proteins gain more stability from the burial and hydrogen bonding of polar groups than from the burial of nonpolar groups (Pace,…”
Get full text

Contribution of Single Tryptophan Residues to the Fluorescence and Stability of Ribonuclease Sa by Alston, Roy W., Urbanikova, Lubica, Sevcik, Jozef, Lasagna, Mauricio, Reinhart, Gregory D., Scholtz, J. Martin, Pace, C. Nick

“…Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other…”
Get full text

Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic by Ilinskaya, Olga N., Dreyer, Florian, Mitkevich, Vladimir A., Shaw, Kevin L., Pace, C. Nick, Makarov, Alexander A.

“…Ribonuclease Sa (pI = 3.5) from Streptomyces aureofaciens and its 3K (D1K, D17K, E41K) (pI = 6.4) and 5K (3K + D25K, E74K) (pI = 10.2) mutants were tested for…”
Get full text

Increasing Protein Conformational Stability by Optimizing β-Turn Sequence by Trevino, Saul R., Schaefer, Stephanie, Scholtz, J. Martin, Pace, C. Nick

“…Protein conformational stability is an important concern in many fields. Here, we describe a strategy for significantly increasing conformational stability by…”
Get full text