Search Results - "PUNT, Peter J"
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Metabolic specialization in itaconic acid production: a tale of two fungi
Published in Current opinion in biotechnology (01-04-2020)“…[Display omitted] •Aspergillus and Ustilago produce itaconic acid by very different pathways.•Transporters play a central role in fungal organic acid…”
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Novel Design of an α-Amylase with an N-Terminal CBM20 in Aspergillus niger Improves Binding and Processing of a Broad Range of Starches
Published in Molecules (Basel, Switzerland) (27-06-2023)“…In the starch processing industry including the food and pharmaceutical industries, α-amylase is an important enzyme that hydrolyses the α-1,4 glycosidic bonds…”
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Carbohydrate Binding Modules: Diversity of Domain Architecture in Amylases and Cellulases From Filamentous Microorganisms
Published in Frontiers in bioengineering and biotechnology (31-07-2020)“…Enzymatic degradation of abundant renewable polysaccharides such as cellulose and starch is a field that has the attention of both the industrial and…”
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The interplay between transport and metabolism in fungal itaconic acid production
Published in Fungal genetics and biology (01-04-2019)“…•Comparison of transporters from A. terreus and U. maydis.•Exporter exchange affects relative levels of itaconate and (S)-2-hydroxyparaconate.•MttA from A…”
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Deciphering domain structures of Aspergillus and Streptomyces GH3-β-Glucosidases: a screening system for enzyme engineering and biotechnological applications
Published in BMC research notes (10-09-2024)“…The glycoside hydrolase family 3 (GH3) β-glucosidases from filamentous fungi are crucial industrial enzymes facilitating the complete degradation of…”
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Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi
Published in Fungal biology and biotechnology (21-09-2019)“…CRISPR/Cas9 mediated genome editing has expedited the way of constructing multiple gene alterations in filamentous fungi, whereas traditional methods are…”
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Characterizing MttA as a mitochondrial cis-aconitic acid transporter by metabolic engineering
Published in Metabolic engineering (01-05-2016)“…The mitochondrial carrier protein MttA is involved in the biosynthesis of itaconic acid in Aspergillus terreus. In this paper, the transport specificity of…”
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Streptomyces small laccase expressed in Aspergillus Niger as a new addition for the lignocellulose bioconversion toolbox
Published in Fungal biology and biotechnology (02-09-2024)“…Laccases are multi-copper oxidases that are usually composed of three Cu-oxidase domains. Domains one and three house the copper binding sites, and the second…”
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Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production
Published in BMC biotechnology (04-11-2019)“…In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters…”
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Identification of a Classical Mutant in the Industrial Host Aspergillus niger by Systems Genetics: LaeA Is Required for Citric Acid Production and Regulates the Formation of Some Secondary Metabolites
Published in G3 : genes - genomes - genetics (01-01-2016)“…The asexual filamentous fungus Aspergillus niger is an important industrial cell factory for citric acid production. In this study, we genetically…”
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Identifying inhibitory compounds in lignocellulosic biomass hydrolysates using an exometabolomics approach
Published in BMC biotechnology (21-03-2014)“…Inhibitors are formed that reduce the fermentation performance of fermenting yeast during the pretreatment process of lignocellulosic biomass. An…”
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Identification of novel citramalate biosynthesis pathways in Aspergillus niger
Published in Fungal biology and biotechnology (19-11-2019)“…The filamentous fungus is frequently used for industrial production of fermentative products such as enzymes, proteins and biochemicals. Notable examples of…”
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An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data
Published in BMC genomics (19-10-2010)“…The ecological niche occupied by a fungal species, its pathogenicity and its usefulness as a microbial cell factory to a large degree depends on its secretome…”
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Itaconic acid degradation in Aspergillus niger : the role of unexpected bioconversion pathways
Published in Fungal biology and biotechnology (04-01-2019)“…Itaconic acid (IA), a C5-dicarboxylic acid, has previously been identified as one of the top twelve biochemicals that can be produced by biotechnological…”
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Profile Comparer Extended: phylogeny of lytic polysaccharide monooxygenase families using profile hidden Markov model alignments [version 1; peer review: 1 approved, 2 approved with reservations]
Published in F1000 research (2019)“…Insight into the inter- and intra-family relationship of protein families is important, since it can aid understanding of substrate specificity evolution and…”
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A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan
Published in Cell surface (Amsterdam) (01-12-2020)“…•Aspergillus niger has seven chitin transglycosylation (crh) gene orthologues.•All seven crh genes (crhA-G) are dispensable for growth and…”
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Characterization of the Aspergillus niger prtT, a unique regulator of extracellular protease encoding genes
Published in Fungal genetics and biology (01-12-2008)“…Expression of several Aspergillus niger genes encoding major secreted, but not vacuolar, protease genes including the major acid protease gene pepA, was shown…”
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Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger
Published in Frontiers in fungal biology (25-05-2021)“…Tannic acid, a hydrolysable gallotannin present in plant tissues, consists of a central glucose molecule esterified with gallic acid molecules. Some…”
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Utilization of ferulic acid in Aspergillus niger requires the transcription factor FarA and a newly identified Far-like protein (FarD) that lacks the canonical Zn(II)2Cys6 domain
Published in Frontiers in fungal biology (08-11-2022)“…The feruloyl esterase B gene ( faeB ) is specifically induced by hydroxycinnamic acids (e.g. ferulic acid, caffeic acid and coumaric acid) but the…”
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Heme biosynthesis and its regulation: towards understanding and improvement of heme biosynthesis in filamentous fungi
Published in Applied microbiology and biotechnology (01-08-2011)“…Heme biosynthesis in fungal host strains has acquired considerable interest in relation to the production of secreted heme-containing peroxidases. Class II…”
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