Search Results - "PRZYBYCIEN, T. M"

Development of a novel gene delivery scaffold utilizing colloidal gold-polyethylenimine conjugates for DNA condensation by SULLIVAN, M. M, GREEN, J. J, PRZYBYCIEN, T. M

“…We have developed a novel gene delivery scaffold based on DNA plasmid condensation with colloidal gold/polyethylenimine conjugates. This scaffold system was…”
Get full text

Precipitation of proteins in supercritical carbon dioxide by Winters, M A, Knutson, B L, Debenedetti, P G, Sparks, H G, Przybycien, T M, Stevenson, C L, Prestrelski, S J

“…Supercritical CO2 was used as an antisolvent to form protein particles that exhibited minimal loss of activity upon reconstitution. Organic protein solutions…”
Get more information

Alternative bioseparation operations: life beyond packed-bed chromatography by Przybycien, Todd M, Pujar, Narahari S, Steele, Landon M

“…Chromatography is undoubtedly the workhorse of downstream processes, affording high resolution for bioseparations. At the same time, it has the notoriety of…”
Get full text

Self-interaction chromatography: A tool for the study of protein-protein interactions in bioprocessing environments by Patro, Sugunakar Y., Przybycien, Todd M.

“…We describe a new protein characterization technique called self‐interaction chromatography (SIC), which exploits the specificity of protein–protein…”
Get full text

Protein purification with vapor-phase carbon dioxide by Winters, Michael A., Frankel, David Z., Debenedetti, Pablo G., Carey, Jannette, Devaney, Margaret, Przybycien, Todd M.

“…Gaseous CO2 was used as an antisolvent to induce the fractional precipitation of alkaline phosphatase, insulin, lysozyme, ribonuclease, trypsin, and their…”
Get full text

Irreversible Inactivation of Interleukin 2 in a Pump-Based Delivery Environment by Tzannis, Stelios T., William J. M. Hrushesky, Wood, Patricia A., Przybycien, Todd M.

“…The physical stability of pharmaceutical proteins in delivery environments is a critical determinant of biological potency and treatment efficacy, and yet it…”
Get full text

Secondary structure characterization of microparticulate insulin powders by Yeo, S D, Debendetti, P G, Patro, S Y, Przybycien, T M

“…The secondary structure content of microparticulate insulin powders produced by the supercritical antisolvent (SAS) precipitation technique was investigated…”
Get more information

Long-term and high-temperature storage of supercritically-processed microparticulate protein powders by WINTERS, M. A, DEBENEDETTI, P. G, CAREY, J, SPARKS, H. G, SANE, S. U, PRZYBYCIEN, T. M

“…The long-term and high-temperature storage of dry, micron-sized particles of lysozyme, trypsin, and insulin was investigated. Subsequent to using supercritical…”
Get full text

Simulations of kinetically irreversible protein aggregate structure by Patro, S.Y., Przybycien, T.M.

“…We have simulated the structure of kinetically irreversible protein aggregates in two-dimensional space using a lattice-based Monte-Carlo routine. Our model…”
Get full text

A Holistic Approach to Protein Secondary Structure Characterization Using Amide I Band Raman Spectroscopy by Sane, Samir U., Cramer, Steven M., Przybycien, Todd M.

“…We have developed a holistic protein structure estimation technique using amide I band Raman spectroscopy. This technique combines the superposition of…”
Get full text

Protein structure perturbations on chromatographic surfaces by Sane, Samir U, Cramer, Steven M, Przybycien, Todd M

“…Amide I band Raman spectroscopy was used to quantify the secondary structure contents of proteins adsorbed on ion-exchange and reversed-phase materials…”
Get full text

Secondary structure perturbations in salt-induced protein precipitates by Przybycien, T M, Bailey, J E

“…The secondary structure implications of precipitation induced by a chaotropic salt, KSCN, and a structure stabilizing salt, Na2SO4, were studied for twelve…”
Get more information

Structure-function relationships in the inorganic salt-induced precipitation of alpha-chymotrypsin by Przybycien, T M, Bailey, J E

“…alpha-Chymotrypsin (alpha CT) was used as a model protein to study the effects of salt-induced precipitation on protein conformation. Process parameters…”
Get more information

Protein-protein interactions as a means of purification by Przybycien, Todd M

“…Hetero-association-based separations are characterized by great specificity and large protein—protein interaction energies and are well suited to application…”
Get full text

Simulations of reversible protein aggregate and crystal structure by Patro, S.Y., Przybycien, T.M.

“…We simulated the structure of reversible protein aggregates as a function of protein surface characteristics, protein-protein interaction energies, and the…”
Get full text

Secondary structure characterization of beta-lactamase inclusion bodies by Przybycien, T M, Dunn, J P, Valax, P, Georgiou, G

“…The secondary structure of proteins in E. coli inclusion bodies was investigated via Raman spectroscopy. Inclusion bodies were purified from cells expressing…”
Get more information

Adsorption of a Formulated Protein on a Drug Delivery Device Surface by Tzannis, Stelios T., Hrushesky, William J.M., Wood, Patricia A., Przybycien, Todd M.

“…The interactions between therapeutic proteins and delivery device surfaces may have a significant impact on the efficacy of a recombinant protein-based…”
Get full text

A Prototype Electrochemical Chromatographic Column for Use with Proteins by Lam, Philippe, Kumar, Kartik, Wnek, Gary E, Przybycien, Todd M

“…We developed electrochemical hardware and media targeted for protein chromatography. Two types of stationary phases were investigated. The first comprised…”
Get full text

Separation of PEGylated from unmodified ribonuclease A using sepharose media by Cisneros-Ruiz, Mayra, Mayolo-Deloisa, Karla, Przybycien, Todd M., Rito-Palomares, Marco

“…PEGylation, used to mitigate some of the problems that affect the effectiveness of therapeutic proteins, often results in a heterogeneous population of…”
Get full text

A Model for Metal Affinity Protein Precipitation by Iyer, Harish V., Przybycien, Todd M.

“…We have modeled the kinetics of the metal affinity aggregation of proteins via a formalism that employs classical gelation theory in conjunction with ion…”
Get full text