Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry

Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry

A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (...

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Bibliographic Details
Published in:Journal of AOAC International Vol. 93; no. 1; pp. 141 - 149
Main Authors: SHAH, Hiten J, KUNDLIK, Mohan L, KAKAD, Abhijit, PATEL, Nitesh K, PANDYA, Ankit, KHATRI, Vanita, PRAJAPATI, Shivkumat, SUBBAIAH, Gunta, PATEL, Chhagan N
Format: Journal Article
Language:English
Published: Gaithersburg, MD AOAC International 2010
Oxford University Press
Subjects:
Biological and medical sciences
Blood Chemical Analysis - methods
Blood plasma
Chemical properties
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - statistics & numerical data
Chromatography, Reverse-Phase - methods
Chromatography, Reverse-Phase - statistics & numerical data
Composition
Drug Stability
Food industries
Fundamental and applied biological sciences. Psychology
Humans
Imipramine - blood
Imipramine - standards
Liquid chromatography
Mass spectrometry
Methods
Paroxetine
Paroxetine - blood
Physiological aspects
Reference Standards
Serotonin Uptake Inhibitors - blood
Spectrometry, Mass, Electrospray Ionization - methods
Spectrometry, Mass, Electrospray Ionization - statistics & numerical data
Tandem Mass Spectrometry - methods
Tandem Mass Spectrometry - statistics & numerical data
Technology application
India
Human
Paroxetine
Liquid chromatography
Mass spectrometry
Electrospray
Quantitative analysis
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Summary:A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (v/v) formic acid in methanol-acetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.5-80.0 ng/mL with r > or = 0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95%. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects.
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ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/93.1.141