Search Results - "PLÜCKTHUN, Andreas"

Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy by Pluckthun, Andreas

“…Designed ankyrin repeat proteins (DARPins) can recognize targets with specificities and affinities that equal or surpass those of antibodies, but because of…”
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Reproducibility: Standardize antibodies used in research by Bradbury, Andrew, Plückthun, Andreas

“…To save millions of dollars and dramatically improve reproducibility, protein-binding reagents must be defined by their sequences and produced as recombinant…”
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DARPins bind their cytosolic targets after having been translocated through the protective antigen pore of anthrax toxin by Becker, Lukas, Plückthun, Andreas

“…Intracellular protein–protein interactions in aberrant signaling pathways have emerged as a prime target in several diseases, particularly cancer. Since many…”
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Assessment of ab initio models of protein complexes by molecular dynamics by Radom, Filip, Plückthun, Andreas, Paci, Emanuele

“…Determining how proteins interact to form stable complexes is of crucial importance, for example in the development of novel therapeutics. Computational…”
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DARPins and other repeat protein scaffolds: advances in engineering and applications by Boersma, Ykelien L, Plückthun, Andreas

“…Highlights ► Designed repeat protein scaffolds form a promising alternative to antibodies. ► These proteins lack disulfides, allowing soluble expression in the…”
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High-Throughput Quantification of Surface Protein Internalization and Degradation by Stüber, Jakob C, Kast, Florian, Plückthun, Andreas

“…Cell surface proteins are key regulators of fundamental cellular processes and, therefore, often at the root of human diseases. Thus, a large number of…”
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Crystal structure of the α1B-adrenergic receptor reveals molecular determinants of selective ligand recognition by Deluigi, Mattia, Morstein, Lena, Schuster, Matthias, Klenk, Christoph, Merklinger, Lisa, Cridge, Riley R., de Zhang, Lazarus A., Klipp, Alexander, Vacca, Santiago, Vaid, Tasneem M., Mittl, Peer R. E., Egloff, Pascal, Eberle, Stefanie A., Zerbe, Oliver, Chalmers, David K., Scott, Daniel J., Plückthun, Andreas

“…α-adrenergic receptors (αARs) are G protein-coupled receptors that regulate vital functions of the cardiovascular and nervous systems. The therapeutic…”
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In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex by Kumar, Abhinav, Plückthun, Andreas

“…G protein coupled receptors (GPCRs) are central players in recognizing a variety of stimuli to mediate diverse cellular responses. This myriad of functions is…”
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Engineering of Challenging G Protein-Coupled Receptors for Structure Determination and Biophysical Studies by Waltenspühl, Yann, Ehrenmann, Janosch, Klenk, Christoph, Plückthun, Andreas

“…Membrane proteins such as G protein-coupled receptors (GPCRs) exert fundamental biological functions and are involved in a multitude of physiological…”
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High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist by Ehrenmann, Janosch, Schöppe, Jendrik, Klenk, Christoph, Rappas, Mathieu, Kummer, Lutz, Doré, Andrew S., Plückthun, Andreas

“…Parathyroid hormone 1 receptor (PTH1R) is a class B multidomain G-protein-coupled receptor (GPCR) that controls calcium homeostasis. Two endogenous peptide…”
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Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists by Schöppe, Jendrik, Ehrenmann, Janosch, Klenk, Christoph, Rucktooa, Prakash, Schütz, Marco, Doré, Andrew S., Plückthun, Andreas

“…Neurokinins (or tachykinins) are peptides that modulate a wide variety of human physiology through the neurokinin G protein-coupled receptor family, implicated…”
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Evolution of Three Human GPCRs for Higher Expression and Stability by Dodevski, Igor, Plückthun, Andreas

“…We recently developed a display method for the directed evolution of integral membrane proteins in the inner membrane of Escherichia coli for higher expression…”
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Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems by Stüber, Jakob C, Plückthun, Andreas

“…Fluorescent labeling of specific cell-surface proteins enables a manifold of techniques to study their function in health and disease. A frequently cited…”
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PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling by Yen, Hsin-Yung, Hoi, Kin Kuan, Liko, Idlir, Hedger, George, Horrell, Michael R., Song, Wanling, Wu, Di, Heine, Philipp, Warne, Tony, Lee, Yang, Carpenter, Byron, Plückthun, Andreas, Tate, Christopher G., Sansom, Mark S. P., Robinson, Carol V.

“…G-protein-coupled receptors (GPCRs) are involved in many physiological processes and are therefore key drug targets 1 . Although detailed structural…”
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Peptide binding affinity redistributes preassembled repeat protein fragments by Michel, Erich, Plückthun, Andreas, Zerbe, Oliver

“…Designed armadillo repeat proteins (dArmRPs) are modular peptide binders composed of N- and C-terminal capping repeats Y and A and a variable number of…”
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Covalently circularized nanodiscs for studying membrane proteins and viral entry by Nasr, Mahmoud L, Baptista, Diego, Strauss, Mike, Sun, Zhen-Yu J, Grigoriu, Simina, Huser, Sonja, Plückthun, Andreas, Hagn, Franz, Walz, Thomas, Hogle, James M, Wagner, Gerhard

“…Membrane proteins can be stabilized in a native-like setting using lipid-bilayer-based nanodiscs encircled by a membrane scaffold protein. Covalently…”
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An automated iterative approach for protein structure refinement using pseudocontact shifts by Cucuzza, Stefano, Güntert, Peter, Plückthun, Andreas, Zerbe, Oliver

“…NMR structure calculation using NOE-derived distance restraints requires a considerable number of assignments of both backbone and sidechains resonances, often…”
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Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc by Matsuda, Shinya, Schaefer, Jonas V., Mii, Yusuke, Hori, Yutaro, Bieli, Dimitri, Taira, Masanori, Plückthun, Andreas, Affolter, Markus

“…How morphogen gradients control patterning and growth in developing tissues remains largely unknown due to lack of tools manipulating morphogen gradients…”
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Structural basis for the activation and ligand recognition of the human oxytocin receptor by Waltenspühl, Yann, Ehrenmann, Janosch, Vacca, Santiago, Thom, Cristian, Medalia, Ohad, Plückthun, Andreas

“…The small cyclic neuropeptide hormone oxytocin (OT) and its cognate receptor play a central role in the regulation of social behaviour and sexual reproduction…”
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Rapid selection of high-affinity binders using ribosome display by Dreier, Birgit, Plückthun, Andreas

“…Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It…”
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