Mucosal protective effects of vitamin E and misoprostol during acute radiation-induced enteritis in rats

Cytotoxic effects of ionizing radiation on gastrointestinal epithelium may be mediated by oxygen free radicals. Therapeutic intervention directed toward oxidant scavenging and increasing tissue oxygen tension may provide a novel approach to management. We investigated the effects of a nonenzymatic o...

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Bibliographic Details
Published in:	Digestive diseases and sciences Vol. 37; no. 2; pp. 205 - 214
Main Authors:	EMPEY, L. R, PAPP, J. D, JEWELL, L. D, FEDORAK, R. N
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-02-1992
Subjects:	Acute Disease Animals Biological and medical sciences Colon - metabolism Colon - pathology Colon - radiation effects Digestive system Enteritis - pathology Enteritis - prevention & control Ileum - metabolism Ileum - pathology Ileum - radiation effects Intestinal Absorption - drug effects Intestinal Mucosa - drug effects Intestinal Mucosa - radiation effects Jejunum - metabolism Jejunum - pathology Jejunum - radiation effects Medical sciences Misoprostol - pharmacology Pharmacology. Drug treatments Radiation Injuries, Experimental - pathology Radiation Injuries, Experimental - prevention & control Random Allocation Rats Vitamin E - pharmacology Rat Toxicity Arachidonic acid derivatives Rodentia Vitamin Prostaglandin E1 α-Tocopherol Vertebrata Chemotherapy Ionizing irradiation Mammalia Treatment Analog Animal Antisecretory agent Digestive diseases Intestinal disease Enteritis
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Summary:	Cytotoxic effects of ionizing radiation on gastrointestinal epithelium may be mediated by oxygen free radicals. Therapeutic intervention directed toward oxidant scavenging and increasing tissue oxygen tension may provide a novel approach to management. We investigated the effects of a nonenzymatic oxygen radical scavenger (vitamin E) and an exogenous PGE1 analog known to increase mucosal blood flow (misoprostol) on acute radiation enteritis. Rats were pretreated with: (1) vitamin E, (2) misoprostol, or (3) a combination of both agents prior to 10 Gy abdominal radiation. Three days following irradiation, net fluid absorption using in vivo isolated loops, mucosal histology, and mucosal morphometry using a computerized videoplan were determined in jejunum, ileum, and colon. Nonirradiated control intestine demonstrated net fluid absorption in all segments, which was not altered by vitamin E and/or misoprostol treatment. Irradiation significantly reduced net fluid absorption in jejunum, ileum, and colon. Vitamin E administered prior to irradiation maintained jejunal, ileal, and colonic fluid absorption near control levels. In contrast misoprostol or a combination of vitamin E and misoprostol did not provide protection against the injury caused by abdominal irradiation. Alterations in intestinal fluid absorption occurred without significant changes in histologic or morphometric appearance. In conclusion, ionizing radiation reduces in vivo intestinal fluid absorption without significant changes in histologic or morphometric appearance. Treatment with vitamin E, but not misoprostol, protects gastrointestinal mucosa against radiation-induced absorptive injury.
ISSN:	0163-2116 1573-2568
DOI:	10.1007/BF01308173