Characterization of two Japanese encephalitis virus strains isolated in Thailand

Two strains of Japanese encephalitis (JE) virus were isolated from a pool of Culex tritaeniorhynchus captured in 1992 and another pool of Cx. vishnui captured in 1993, in Chiang Mai Area, Northern Thailand. These two strains, ThCMAr44/92 and ThCMAr67/93, could not be identified either as Nakayama or...

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Bibliographic Details
Published in:	Archives of virology Vol. 140; no. 9; pp. 1557 - 1575
Main Authors:	ALI, A, IGARASHI, A, PANERU, L. R, HASEBE, F, MORITA, K, TAKAGI, M, SUWONKERD, W, TSUDA, Y, WADA, Y
Format:	Journal Article
Language:	English
Published:	Wien Springer 01-09-1995 New York, NY
Subjects:	Animals Base Sequence Biological and medical sciences Culex - microbiology DNA Primers - chemistry Encephalitis Virus, Japanese - genetics Encephalitis Virus, Japanese - isolation & purification Encephalitis, Japanese - microbiology Fundamental and applied biological sciences. Psychology Genes, Viral Insect Vectors - microbiology Microbiology Molecular Sequence Data Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA, Viral - analysis RNA, Viral - genetics Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Thailand Viral Proteins - genetics Viral Structural Proteins - genetics Virology Thailand Asia Typing Nucleotide sequence Insecta Genotype Identification Flavivirus Epidemiology Virus Culex tritaeniorhynchus Arthropoda Culicidae Japanese encephalitis virus Serotype Flaviviridae Invertebrata Vector Diptera Aminoacid sequence
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Summary:	Two strains of Japanese encephalitis (JE) virus were isolated from a pool of Culex tritaeniorhynchus captured in 1992 and another pool of Cx. vishnui captured in 1993, in Chiang Mai Area, Northern Thailand. These two strains, ThCMAr44/92 and ThCMAr67/93, could not be identified either as Nakayama or JaGAr01 subtype by the hemagglutination-inhibition (HI) and the neutralization (N) tests using immune sera raised against these standard JE virus strains. Reverse transcription-polymerase chain reaction showed the presence of JE-specific conserved sequences in these strains. Sequencing of 240 nucleotides in their PrM gene region identified that these two strains belong to the genotype 1 of JE virus. Nucleotide and encoded amino acid sequences of their envelope glycoprotein gene revealed 98.8 and 99.8% identity, respectively. These two strains shared 77.8 to 87.7% homology in the nucleotide sequence and 90.0 to 98.8% homology in the amino acid sequence with other reported JE strains. Five strain-specific amino acid changes were noted in ThCMAr44/92 strain, while one in ThCMAr67/93. In addition, four common amino acid changes were found in both strains. Thus, the findings indicated that these two strains were structurally different from each other as well as different from all the reported strains which was in agreement with the serological tests by hemagglutination-inhibition and neutralization.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798
DOI:	10.1007/bf01322530