CTNNB1 AND VIMENTIN SWITCH EXPRESSION IN ORAL SQUAMOUS CELL CARCINOMA CELL LINES UNDER CHOLESTEROL DEPLETION

CTNNB1 AND VIMENTIN SWITCH EXPRESSION IN ORAL SQUAMOUS CELL CARCINOMA CELL LINES UNDER CHOLESTEROL DEPLETION

Objective: Evaluating the expression of epithelial marker β-catenin (CTNNB1) and the mesenchymal marker vimentin (VIM) in the oral squamous cell carcinoma (OSCC) cell line SCC-9) under caveolae disruption through different concentrations of Methyl-β-cyclodextrin (MβCD) substance. Study Design: The O...

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Published in:Oral surgery, oral medicine, oral pathology and oral radiology Vol. 130; no. 3; p. e263
Main Authors: SILVA, MONIQUE HEVLIN DIAS DA, NASCIMENTO, REBECA BARROS, SAMPAIO, MARIA GABRIELLY BARRETO, SANTOS, JEAN NUNES DOS, RODRIGUES, MARIA FERNANDA DESTRO, PAIVA, KATIúSCIA BATISTA, XAVIER, FLáVIA CALó DE AQUINO
Format: Journal Article
Language:English
Published: Elsevier Inc 01-09-2020
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Summary:Objective: Evaluating the expression of epithelial marker β-catenin (CTNNB1) and the mesenchymal marker vimentin (VIM) in the oral squamous cell carcinoma (OSCC) cell line SCC-9) under caveolae disruption through different concentrations of Methyl-β-cyclodextrin (MβCD) substance. Study Design: The OSCC cell line SCC-9 was treated with MβCD at different concentrations (7.5 mM, 10 mM, and 15 mM) for 1 hour. After 24 hours of depletion, the gene expression of VIM and CTNNB1 was analyzed by quantitative reverse transcription polymerase chain reaction (RT-qPCR), comparing to untreated SCC-9 cells. Results: Negative expression of VIM was observed at the 10 mM and 15 mM concentrations (mean 0.89 and 0.67, respectively). Gene expression at 15 mM was significantly lower compared with 7.5 mM (mean, 1.39; P value, .029; 1-way analysis of variance [ANOVA]). On the other hand, CTNNB1 was positively expressed by SCC-9 cells after cholesterol depletion with MβCD at all concentrations of 7.5 mM, 10 mM, and 15 mM (mean 3.23, 2.66, and 1.78, respectively), showing a decrease in expression in a dose-dependent manner (P value, .277; 1-way ANOVA).Conclusion: The inversion of expression between the epithelial (CTNNB1) and mesenchymal (VIM) marker after cholesterol depletion by MβCD may reflect biological changes that correspond to the reversal of the epithelial- mesenchymal transition process in OSCC.
ISSN:2212-4403
2212-4411
DOI:10.1016/j.oooo.2020.04.704