Screening for restriction endonucleases in aerobic, thermophilic eubacteria

A total of 216 Icelandic aerobic, heterotrophic, thermophiles belonging to three different genera were screened for type II restriction endonucleases. The frequency of positive strains was 44% for both Thermus and Bacillus but 63% for Rhodothermus. Approximately half of the enzymes from each group w...

Full description

Saved in:

Bibliographic Details
Published in:	Biotechnology techniques Vol. 10; no. 1; pp. 13 - 18
Main Authors:	Hj rleifsd ttir, Sigr dur, P tursd ttir, S lveig K., Korpela, Jaana, Torsti, Anna-Maria, Mattila, Pekka, Kristj nsson, Jakob K.
Format:	Journal Article
Language:	English
Published:	01-01-1996
Subjects:	Bacillus Rhodothermus Thermus
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A total of 216 Icelandic aerobic, heterotrophic, thermophiles belonging to three different genera were screened for type II restriction endonucleases. The frequency of positive strains was 44% for both Thermus and Bacillus but 63% for Rhodothermus. Approximately half of the enzymes from each group were characterized and a total of 14 different restriction enzymes were found. In all cases they were isoschizomers of known enzymes. Thermus contained 9 different types, Bacillus 6 and Rhodothermus had 3. This is the first time that isoschizomers of BspEI, BgII, EagI and EcoRV are found in Thermus and BstBI and EcoRV are found in Rhodothermus.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0951-208X 1573-6784
DOI:	10.1007/BF00161077