Lactobacillus spp. impair the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells

Objectives The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival....

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Published in:	Biotechnology letters Vol. 40; no. 8; pp. 1237 - 1244
Main Authors:	Castellano, P., Pérez Ibarreche, M., Longo Borges, L., Niño Arias, F. C., Ross, G. R., De Martinis, E. C. Pereira
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-08-2018 Springer Nature B.V
Subjects:	Adhesion Alginates Alginic acid Antimicrobial activity Applied Microbiology Bacteria Bacterial Adhesion - drug effects Bacterial Adhesion - physiology Beads Bile Acids and Salts - metabolism Bile salts Biochemistry Biomedical and Life Sciences Biotechnology Caco-2 Cells Chenodeoxycholic acid Encapsulation Food processing industry Humans Intestine Lactobacillus Lactobacillus - metabolism Lactobacillus - physiology Life Sciences Listeria Listeria monocytogenes Listeria monocytogenes - metabolism Listeria monocytogenes - pathogenicity Listeria monocytogenes - physiology Microbial Interactions - physiology Microbiology Microencapsulation Original Research Paper Probiotics Probiotics - pharmacology Salts Strains (organisms) Substrates Survival Probiotic Adhesion Invasion L. monocytogenes
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Summary:	Objectives The objective of this study was to evaluate the ability of Lactobacillus curvatus CRL705, CRL1532, and CRL1533 and Lactobacillus sakei CRL1613 to survive under simulated gastrointestinal conditions. Moreover, a microencapsulation approach was proposed to improve gastrointestinal survival. Finally, experiments were performed to demonstrate that Lactobacill us spp. can modulate the ability of Listeria monocytogenes FBUNT to adhere to and invade Caco-2 cells. Results Lactobacillus strains were encapsulated in alginate beads to enhance the survival of bacteria under in vitro gastrointestinal conditions. All strains hydrolyzed bile salts using chenodeoxycholic acid as a substrate and adhered to Caco-2 cells. Cell-free supernatants (CFSs) showed antimicrobial activity against L. monocytogenes as demonstrated by agar diffusion assays. The average percentages of L. monocytogenes adhesion decreased from 67.74 to 41.75 and 38.7% in the presence of 50 and 90% (v/v), respectively, for all CFSs tested. The highest concentrations of CFSs completely inhibited the L. monocytogenes invasion of Caco-2 cells. Conclusions The studied Lactobacillus strains have protective effects against the adhesion and invasion of L. monocytogenes FBUNT. Alginate encapsulation of these bacteria improved gastrointestinal tolerance such that they could be further studied as potential probiotics against intestinal pathogenic bacteria.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0141-5492 1573-6776
DOI:	10.1007/s10529-018-2572-x